增殖细胞核抗原、Ki-67在肝内胆管癌及肝内胆管结石胆管中的表达及意义  

Expression of proliferating cell nuclear antigen and Ki-67 in intrahepatic cholangiocarcinoma and hepatolithiasis tissue

作　　者：张文华[1] 唐瑞峰[1] 白建国[1] 孙超[1] 尚军峰 于国栋[1] 姚红亮[1] 

机构地区：[1]河北医科大学第四医院肝胆外科,河北石家庄050011

出　　处：《临床荟萃》2014年第1期35-37,F0003,共4页Clinical Focus

基　　金：河北省普通高等学校强势特色学科肿瘤学建设经费资助项目(冀高教[2005]52号)

摘　　要：目的研究分析增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)及Ki-67在肝内胆管癌组织、肝内胆管结石胆管组织中的表达,探讨肝内胆管结石与肝内胆管癌的关系。方法利用免疫组织化学染色法检测肝内胆管癌组织(n=25)、肝内胆管结石胆管组织(n=20)以及正常肝内胆管组织(n=30)中PCNA及Ki-67的表达,结合临床病理特征进行统计学分析。结果 PCNA在肝内胆管癌、肝内胆管结石及正常肝内胆管组织中的表达率依次为96.0%(24/25)、65.0%(13/20)、6.7%(2/30);Ki-67的表达率依次为68.0%(17/25)、35.0%(7/20)、3.3%(1/30),差异有统计学意义(P<0.05)。Ki-67在高-中分化及低分化的肝内胆管癌组织中的表达差异有统计学意义(P<0.05)。PCNA及Ki-67在肝内胆管癌和肝内胆管结石胆管组织中的表达无相关性(P>0.05)。结论 PCNA及Ki-67在正常肝内胆管组织、肝内胆管结石胆管组织、肝内胆管癌组织中的表达率逐渐增高,提示肝内胆管结石可能是肝内胆管癌的病因。PCNA及Ki-67在肝内胆管癌和肝内胆管结石胆管组织中的表达无相关性。Objective To study the expression of proliferating cell nuclear antigen （PCNA） and Ki-67 in intrahepatic cholangiocarcinoma and hepatolithiasis tissues,and to explore the relationship between hepatolithiasis and intrahepatic cholangiocarcinoma. Methods The expressions of PCNA and Ki-67 were examined by immunohistochemisrry in intrahepatic cholangioearcinoma tissues（ n =25）, hepatolithiasis tissue（ n =20） and normal intrahepatic bile duet tissue （n = 30）. Results ＇The expression rates of PCNA in intrahepatic ch01angiocarcin0ma tissue, hepatolithiasis tissue and normal intrahepatic bile duct tissue were 96.0 %（24/25）, 65.0 % （13/20） and 6.7 % （2/ 30）,respectively, the difference were significant （P 〈0.05）. The expression rates of Ki-67 were 68.0%（17/25）, 35.0% （7/20） and 3.3% （1/30） ,respectively,there were statistic significance （ P 〈0.05）. The PCNA expression rates in intrahepatic cholangiocarcinoma well-moderately and poorly differentiated were not significant （ P 〉0. 05） ,however there were statistical significance in the Ki-67 expression rates （ P 〈0.05）. pCNA and Ki-67 expression were not correlated in intrahepatic cholangiocarcinoma and hepatolithiasis tissues （ P 〉 0.05）. Conclusion PCNA and Ki-67 expression rates were increased gradually in normal intrahepatic biie duct tissue, hepatolithiasis tissue and intrahepatic cholangiocarcinoma tissue,which suggested that hepatolithiasis may be the cause of intrahepatic cholangiocarcinoma. PCNA and Ki-67 expressions in intrahepatic cholangiocarcinoma and hepatolithiasis tissue＇ were not closely related.

关 键 词：胆管肿瘤 胆结石 增殖细胞核抗原 KI-67抗原 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]