自分泌乙酰胆碱对大鼠胸腺组织细胞增殖及分化的影响  

Effect of non-neuronal acetylcholine on proliferation and differenation of rat thymus cells

作　　者：李永丽[1] 穆上 高峰[3] 赵莹[3] 陈志勇[2] 张清勇[2,4] 

机构地区：[1]河南省人民医院郑州大学人民医院放射科,郑州450003 [2]郑州大学第二附属医院 [3]郑州大学医药科学研究院神经免疫实验室 [4]郑州大学生物工程研究所

出　　处：《中华实验外科杂志》2014年第1期114-116,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30570625）

摘　　要：目的 观察自分泌乙酰胆碱（ACh）对大鼠胸腺组织细胞增殖和分化的影响.方法 取10只大鼠胸腺组织细胞进行体外培养,根据所加试剂分为A组（空白对照）、B组（阿托品）、C组（维库溴铵）及D组（阿托品±维库溴铵）,细胞计数试剂盒（CCK-8）法测各组细胞活力,流式细胞术分析T细胞亚群.结果 B组吸光度（A）值（0.341 ±0.007）低于其他各组（P＜0.05）；C组A值（0.368±0.010）高于其他各组（P＜0.05）,A、D两组差异无统计学意义（P＞0.05）.流式细胞术分析T细胞亚群,各组之间CD3＋ CD4＋细胞及CD3＋ CD8＋细胞所占细胞总数比例差异无统计学意义（P＞0.05）；CD3＋ CD4＋细胞及CD3＋ CD8＋细胞数量之比各组间差异无统计学意义（P＞0.05）.结论 自分泌ACh对大鼠胸腺组织细胞有双向调节作用,可通过毒蕈碱型胆碱能受体提高细胞活力,也可以通过烟碱型胆碱能受体降低细胞活力,其对胸腺细胞的分化方向无明显的倾向性.Objective To explore the effect of non-neuronal acetylcholine on the viability and differentiation of rat thymus cells.Methods The thymus cells were isolated from 10 rats,and cultured in vitro.The cells were divided into 4 groups：group A,control; group B,atropine; group C,vecuronium bromide; group D,atropine and vecuronium bromide.The viability was detected by using cell counting kit-8,and the differentiation by flow cytometry.Results The viability in group B was significantly lower than in the rest groups （P 〈 0.05）.The viability in group C was significantly higher than in the rest groups （P 〈 0.05）.There was no significant difference in the viability between group A and group D.The proportion of CD3 ＋ CD4 ＋ T cells and CD3 ＋ CD8 ＋ T cells showed no significant difference among the 4 groups （P 〉 0.05）.The ratio of CD3 ＋ CD4 ＋ T cells to CD3 ＋ CD8 ＋ T cells showed no significant difference among the 4 group （P 〉 0.05）.Conclusion ACh acts on the thymus cells in two opposite ways.It can enhance the viability of the cells,stimulating muscarinic receptors.In reverse,it can decrease the viability,stimulating nicotinic receptors.The experiment doesn＆#39; t show evidence that ACh have effect on the differen-tiation of neither CD3 ＋ CD4 ＋ T cells nor CD3 ＋ CD8 ＋ T cells.

关 键 词：非神经源性乙酰胆碱 胸腺细胞 细胞活力 细胞分化 

分 类 号：R363[医药卫生—病理学]