姜黄素对骨肉瘤MG-63细胞增殖的影响和抑制作用  

Inhibitory effects of curcumin on growth of human osteosarcoma cell line MG-63 cells

作　　者：王燕 补蔚萍 谢宏 曲爱华 刘军[4] 

机构地区：[1]第四军医大学第二附属医院疼痛生物研究所,西安710038 [2]总参管理保障部北极寺门诊部 [3]解放军94595部队医院 [4]兰州军区总医院

出　　处：《中华实验外科杂志》2014年第1期135-137,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81270170）;西京医院学科助推计划资助项目（XJZT09M16）

摘　　要：目的 观察姜黄素（Cur）对人成骨肉瘤细胞株MG-63增殖的影响和抑制作用.方法 不同浓度的Cur作用于体外培养的MG-63细胞,倒置显微镜下观察MG-63细胞的形态学变化,采用噻唑蓝（MTT）法检测不同浓度Cur对MG-63存活率的影响,细胞计数试剂-8（CCK-8）法检测Cur对MG-63细胞增殖抑制能力的影响,膜联蛋白V（Annexin V）/碘化丙锭（PI）双染法标记检测细胞凋亡,黏附实验测定细胞黏附能力,并检测培养液中乳酸脱氢酶（LDH）释放量.结果 MTT法检测经Cur作用后的MG-63细胞存活率以及细胞黏附能力明显下降,LDH的增加提示MG-63细胞死亡率上升；Cur可抑制骨肉瘤细胞株MG-63细胞增殖,50.0 μmol/L的Cur作用12h后,细胞抑制率为47.23％,24 h以后为65.23％.Cur 12.5、25.0、50.0、100.0μmol/L组作用24h后,MG-63细胞的凋亡率分别为（11.24±0.07）％、（28.23±0.35）％、（50.21±1.71）％、（41.22±1.85）％,不同浓度的Cur之间的实验结果差异有统计学意义（P＜0.05）.结论 Cur能够抑制MG-63细胞增殖,Cur浓度从12.5 μmol/L增加到25.0 μmol/L,其对MG-63细胞24 h时生长抑制率由（13.56±1.88）％增至（48.27±2.32）％,50.0 μmol/L组24 h生长抑制率为65.23％.50.0μmol/L的Cur对MG-63细胞诱导凋亡作用最明显,作用24 h后,细胞凋亡率为（50.21±1.71）％.Objective To investigate the inhibitory effects of curcumin （Cur） on proliferation and apopotosis of human osteosarcoma cell line MG-63 cells.Methods The cultured MG-63 cells were treated with Cur （12.5,25.0,50.0,100.0 μmol/L） for 12 h.The morphologic changes of MG-63 cells were observed by inverted microscope.The survival rate of MG-63 cells was measured by using methyl thiazol tetrazolium （MTT） assay.Cell counting of kit-8 （CCK-8） assay was used to test the growth inhibitory rate.Annexin V/propidium iodide （PI） staining was applied to detect the apoptosis.The survival ratios and the adhesion capacity of MG-63 were detected except the lactate dehydrogenase （LDH） releasing.Results Different concentrations of Cur （12.5,25.0,50.0,100.0 μmol/L） observed in this study have significant effects on the survival ratio of MG-63.The Cur could significantly inhibit the growth of MG-63 in a dose and time dependent manner and induce G2/M Phase cell cycle arrest.After 12 h,cell inhibition rate of 47.23%,24 h after the cell was 65.23%.Cur 12.5,25.0,50.0,100.0 μmol/L group for 24 h,MG-63 cell apoptosis rate was （11.24 ± 0.07）%,（28.23 ± 0.35）%,（50.21 ± 1.71）% and （41.22 ±1.85） %,50.0 μmol/L curcumin could induce apoptosis obviously.Conclusion When Cur increased from 12.5 μmol/L to 25.0 μmol/L,its MG-63 cells at 24h growth inhibition rate （13.56 ± 1.88） % to （48.27 ±2.32）%,50.0 μmol/L group 24 h growth inhibition rate to 65.23%.50.0 μmol/L of Cur on the MG-63 cells apoptosis induced by the most obvious,for 24 h,apoptosis rate was （50.21 ± 1.71）%.

关 键 词：姜黄素 骨肉瘤 增殖 

分 类 号：R738.1[医药卫生—肿瘤]