刺梨高效液相色谱指纹图谱研究  

Study on HPLC Fingerprint of Rosa Roxbughii Tratt.

作　　者：周欣[1] 陈华国[1] 张明[1] 杨世林[1] 

机构地区：[1]贵州师范大学天然药物质量控制研究中心、贵州省山地环境信息系统与生态环境保护重点实验室,贵阳550001

出　　处：《医药导报》2014年第1期82-85,共4页Herald of Medicine

基　　金：“十二五”国家科技支撑计划(2011BAC09B01);贵州省国际合作项目(黔科合外G[2012]7024号);贵州省科技创新人才团队建设项目(黔科合人才团队(2011)4008);贵州省教育厅特色重点实验室项目(黔科合KY[2012]005号);贵阳市科技重大专项项目(筑科合同[2012401]社-4号)

摘　　要：目的建立刺梨的高效液相色谱(HPLC)特征图谱测定方法。方法采用反相高效液相色谱法,Hanbon Phecda C18柱(250 mm×4.6 mm,5μm),乙腈(A)-0.05%磷酸溶液(B)为流动相,梯度洗脱,流速1 mL·min-1,检测波长255 nm,柱温:25℃;测定了14批刺梨药材的指纹图谱,应用相似度分析和聚类分析方法对药材进行分类,建立指纹图谱共有模式。结果建立了刺梨药材的指纹图谱测定方法及其特征图谱,不同产地药材图谱具有一定差异性。结论刺梨HPLC特征图谱的建立为药材质量控制提供了科学依据。Objective To establish an HPLC method for determining fingerprint of Rosa roxbughii. Methods The HPLC method was used with Hanbon Phecda C18 column（250 mm × 4.6 mm,5 μm）. The mobile phase consists of acetonitrile （A） and 0.05% phosphoric acid （B） with a gradient elution. The column temperature was set at 25℃, and the detective wavelength was 255 rim. The HPLC fingerprint for 14 batches of Rosa roxbughii was studied. The similarity analysis and cluster analysis were taken to classify the herbs and establish a common pattern of fingerprints. Results A HPLC method for determination of ingredients in Rosa roxburghii tratt and the fingerprint were established. A unique fingerprint was presented for herbs from different areas. Conclusion The establishment of HPLC feature fingerprint of Rosa roxburghii Tratt provides scientific basis for herbs quality control.

关 键 词：刺梨 色谱法 高效液相 特征图谱 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]