高效液相色谱切换波长法同时测定牛蒡子中活性成分的含量  

Determination of Effective Constituents in Fructus Arctii by Wavelength Switching Method of HPLC

作　　者：孙艳涛[1,2] 赵兰英[1] 李婷婷[1] 赵磊[3] 姜大雨[1,2] 

机构地区：[1]吉林师范大学化学学院,四平136000 [2]吉林师范大学环境友好材料制备与应用省部共建教育部重点实验室,四平136000 [3]吉林省四平市食品药品检验所,四平136000

出　　处：《医药导报》2014年第1期100-102,共3页Herald of Medicine

基　　金：吉林省教育厅十二五基金项目(2012177)

摘　　要：目的建立高效液相色谱（HPLC）切换波长法同时测定牛蒡子中3种活性成分含量。方法应用TC．C18色谱柱（4．6mm×250mm，5μm）；流动相为甲醇-0．4％冰醋酸，梯度洗脱；流速1．0mL·min-1；检测波长为280与326nm切换；进样量10μL；温度25℃。结果绿原酸、牛蒡子苷和牛蒡子苷元分别在0．0273—5．4600，0．1500～18．0000和0．0303—6．0600肛g范周内呈良好线性，线性方程分别为Y=1]06．476X-11．452，Y=1026．014X＋20．391和y=287．661X＋4.045；平均加样回收率分别为99．55％，99．42％和99．40％。结论该方法快速、准确、重复性好，可用于牛蒡子中多组分含量的同时测定及质量控制。Objective To establish an HPLC method for simuhaneous determination of three active components in Fructus arctii. Methods The HPLC analysis was carried out on TC-CIs（4.6 mm×250 mm,5μm） column at the temperature of 25 ℃. The mobile phase composed of methanol and 0. 4% glacial acetic acid with gradient elution at a flow rate of 1.0 mL · rain-~. The detection wavelength was 280 nm shifted to 326 nm. The sample loading volume was 10μL. Results The linear range for chlorogenie acid, arctiin, and arctigenin was 0. 027 3-5. 460 0, 0. 150 0- 18. 000 0, and 0. 030 3- 6. 060 μg, respectively. The linear equation was Y= 1 106. 476X- 11. 452, Y= 1 026. 014X＋20. 391, and Y= 287. 661X＋ 4.045, respectively. The average recovery of cblorogenic acid, arctiin, and arctigenin was 99.55%, 99.42% and 99.40%, respectively. Conclusion The method is quick, accurate, and reproducible. It can be used for content determination and quality control for Fructus arctii.

关 键 词：牛蒡子 绿原酸 牛蒡子苷 牛蒡子苷元 

分 类 号：R282.71[医药卫生—中药学] R927.2[医药卫生—中医学]