桉木氨水浸渍预处理响应面优化提高酶解效率  

Pretreatment of Eucalypt Wood with Optimized SAA (Soaking in Aqueous Ammonia) for Conversion of Lignocellulose to Fermentable Sugars

作　　者：霍丹[1] 房桂干[1] 邓拥军[1] 韩善明[1] 沈奎忠 

机构地区：[1]中国林业科学研究院林产化学工业研究所,江苏南京210042

出　　处：《桉树科技》2013年第4期5-10,共6页Eucalypt Science & Technology

基　　金：林业公益性行业科研专项"桉树生态经营及产业升级关键技术研究"(201104003-06)

摘　　要：采用氨水中温浸渍(SAA)方法对桉木进行预处理,以期提高其后续酶水解效率。以响应面法主要预处理条件进行优化,以期筛选出SAA预处理的最优工艺条件。结果表明:最佳预处理工艺为氨用量80%、时间11 h、温度90℃,在此条件下预处理物料的后续酶解转化率可达31.7%。对预处理物料的分析表明:在SAA预处理过程中,可在不损失纤维素的前提下脱除木素51%,而且主要是紫丁香基型和愈疮木基型木素降解溶出,半纤维素主要是木聚糖也有部分降解溶出。木素及半纤维素的降解、纤维素结晶度的降低都有利于后续的酶水解。The methodology of soaking eucalypt wood in aqueous ammonia （SAA） as a pretreatment for enhancing production of fermentable sugars from enzymolysis process was investigated in this study. Various pretreatment conditions including temperature, time and dosage of aqueous-ammonia on the enzymatic digestibility were optimized using response surface methodology. An enzymatic digestibility of 31.7% was obtained when the eucalypt fibers were pretreated at the optimal conditions： 80.0% ammonia; temperature of 90℃; and, a duration of 11 h. The fourier transform infrared spectroscopy （FTIR） analysis indicated that hemicellulose degradation and lignin structure transformation occurred during SAA pretreatment.

关 键 词：氨水 SAA预处理 桉木 酶水解 还原糖 

分 类 号：S781[农业科学—木材科学与技术]