5种不同石斛的多糖与氨基酸及甘露糖的含量比较  

Determination of Polysaccharide,Amino Acid and Mannose Content in Five Kinds of Dendrobii Caulis

作　　者：鲁芹飞[1] 黄松[1,2] 林培[1] 吕书剑 谢剑锋[1] 

机构地区：[1]广州中医药大学,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808

出　　处：《中国实验方剂学杂志》2014年第2期59-62,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技计划项目(2008A030101009);广东省科技计划项目(2011A030100011);广东省教育部产学研结合项目(2010B090400474)

摘　　要：目的:比较不同种石斛部分化学成分的差别。方法:采用苯酚-硫酸法测定样品中多糖含量,茚三酮显色法测定样品中氨基酸的含量,内标法测定样品中甘露糖的含量,通过定量测定,比较5种石斛中总多糖、氨基酸和甘露糖的含量差异。结果:5种不同种类的石斛中多糖、氨基酸、甘露糖的含量存在较大的差异。多糖含量,细茎石斛、金钗石斛、马鞭石斛相近,其中铁皮石斛明显高于其余4种石斛,鼓槌石斛明显低于其余的4种石斛;氨基酸含量,马鞭石斛高于其他4种石斛;甘露糖含量,铁皮石斛明显高于其余4种石斛。结论:5种不同石斛的总多糖、氨基酸和甘露糖的含量存在较大的差异,但就所含的相同化学成分总体而言铁皮石斛的质量好于其他4种石斛。Objective： To compare the chemical compositions in the five kinds of Dendrobii Caulis. Method： Polysaccharide,mannose and amino acid in the five kinds of Dendrobii Caulis were determined and compared. Result： The polysaccharide contents,the amino acid contents and the mannose contents are remarkably different in the five kinds of Dendrobii Caulis. The content of polysaccharide in the Dendrobii Caulis officinnle is highedest in the five kinds of Dendrobii Caulis;the content of amino acid in the Dendrobii Caulis finbristun is higher than other four kinds of Dendrobii Caulis;the mannose contents of Dendrobii Caulis officinnle is much more higher than other kinds. Conclusion： The quantity and contents of polysaccharide, amino acid and the mannose are remarkably different in the five kinds of Dendrobii Caulis. However,Dendrobii Caulis officinnle has a higher quality than other four kinds of Dendrobii Caulis in terms of the same chemical compositions1.

关 键 词：石斛 多糖 氨基酸 甘露糖 

分 类 号：R284.1[医药卫生—中药学]