W/O/W型人参总皂苷复乳的制备与表征  

Preparation and Characterization of W /O /W Total Saponins from Ginseng Radix Et Rhizoma Multiple Emulsion

作　　者：陈新梅[1] 

机构地区：[1]山东中医药大学药学院,济南250355

出　　处：《中国实验方剂学杂志》2014年第3期32-35,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省高等学校科技计划项目(J09LF56)

摘　　要：目的:制备与表征W/O/W型人参总皂苷复乳。方法:采用两步法制备W/O/W人参总皂苷复乳,以离心保留率为综合评价标准,通过正交试验考察乳化剂Ⅰ用量、明胶溶液的质量分数、乳化剂Ⅱ用量及反应温度对复乳处方工艺的影响,同时结合复乳的粒子形态、粒径和粒度分布,筛选最佳处方并对其进行表征。结果:最佳处方工艺为乳化剂Ⅰ用量8%,明胶溶液的质量分数0.25%,乳化剂Ⅱ用量8%,反应温度70℃。人参总皂苷包裹率71.8%,表面张力32.2 mN·m-1,电导率453μs·cm-1。结论:W/O/W型人参总皂苷复乳性质稳定、质量合格,为提高人参总皂苷的口服生物利用度提供参考。Objective： To prepare W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion. Method： W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion was prepared by two-step method, with centrifugation retention rates as comprehensive evaluation index, orthogonal test was used to investigate effects of dosages of emulsifier Ⅰ and Ⅱ , the concentration of gelatin solution and reaction temperature on formulation technology, then combined with particle morphology and particle size distribution to screen optimum formulation and its characterization. Result： Optimum prescription process was as follows： emulsifier Ⅰ and Ⅱ amounts all of 8% , gelatin solution concentration of 0.25% , reaction temperature at 70 ℃ ; Under these conditions, parcle rate of total saponins from Ginseng Radix Et Rhizoma was 71.8% with surface tension of 32.2 mN .m-1 and conductivity of 453 μs .cm-1 Conclusion： These W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion was stable, it could provide a reference for improving oral bioavailability of total saponins from Ginseng Radix Et Rhizoma.

关 键 词：人参总皂苷 W O W型复乳 正交试验 处方工艺 

分 类 号：R283.6[医药卫生—中药学]