硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较  

Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method

作　　者：辛敏[1,2] 刘轩[2] 詹欣[1,2] 黄昀[2] 唐劲天[1,2] 盛军[1,3] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]清华大学工程物理系粒子技术与辐射成像教育部重点实验室,北京100084 [3]云南农业大学普洱茶学教育部重点实验室,昆明650201

出　　处：《中国实验方剂学杂志》2014年第3期62-65,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国博士后基金项目(2013M530603)

摘　　要：目的：比较苯酚-硫酸法与硫酸-紫外法在测定千两茶多糖含量上的差异。方法：千两茶粉经水提醇沉，Sevage法除蛋白后，采用苯酚-硫酸法和硫酸．紫外法测定茶多糖含量。结果：苯酚-硫酸法标准曲线方程为Y：4．7550X-0．0295（R2=0．9904），硫酸-紫外法标准曲线方程为Y=4．7700X-0．0651（R2=0．9931），均在0．02～0．1g·L-1有良好的线性关系。所测茶多糖质量分数分别为2．00％，3．21％，其差异有统计学意义。结论：硫酸-紫外法作为一种多糖检测新手段，可简便测定多糖含量。Objective： To compare differences between phenol-sulfuric acid method and sulfuric acid-UV method to determine polysaccharides content in Qianliang tea. Method： The two methods were employed to determine content of polysaccharides in Qianliang tea after water extracting-alcohol precipitating and sevage assays. Result： The total sugar stand curve equation were Y=4. 755 OX - O. 029 5 （R2 = 0. 990 4） （by sulfuric acid-UV method） and Y = 4. 770 0X - 0. 065 1 （R2 = 0. 993 1 ） （ by phenol-sulfuric acid method） , which showed a good linear relationship range from 0.02 to 0. 1 g ·L-1. The determination of phenol-sulfuric acid method and sulfuric acid-UV method were 2.00% and 3.21% , which was statistically significance. Conclusion： As a new method, sulfuric acid-UV method could be a stable and simple method to measure the polysaccharide.

关 键 词：茶多糖 千两茶 苯酚一硫酸法 硫酸一紫外法 含量测定 

分 类 号：R284.1[医药卫生—中药学]