RNAi靶向沉默CXCR2基因抑制肝癌增殖及转移的实验研究  

Inhibitory effects of antisense targeted silencing CXCR2 gene on proliferation and invasion of Hepatocellular carcinoma

作　　者：谢斌辉[1] 王小农[1] 刘凤恩[1] 何晓[1] 谢元康[1] 

机构地区：[1]赣南医学院第一附属医院普外科,江西省赣州市341000

出　　处：《实用医学杂志》2014年第2期204-207,共4页The Journal of Practical Medicine

基　　金：江西省教育厅青年基金项目(编号:Gjj10232)

摘　　要：目的:研究反义CXCR2基因体外抑制肝癌增殖及转移的作用。方法:应用PT-PCR及Western blot法分别检测正常肝细胞、肝癌细胞以及转染反义CXCR2基因后肝癌细胞的CXCR2 mRNA和蛋白表达水平;采用CCK-8检测转染后肝癌细胞的增殖;以Transwell法检测转染后肝癌细胞的侵袭能力。结果:CXCR2 mRNA和蛋白表达量在肝癌细胞hepG2和正常肝细胞Chang细胞分别是(1.69±0.22、0.63±0.31和1.93±0.25、0.84±0.29),两者相比,肝癌细胞中CXCR2 mRNA和蛋白表达水平均明显升高,差异有统计学意义,(P<0.05)。转染反义CXCR2基因后,肝癌细胞CXCR2 mRNA和蛋白表达下降;CCK-8检测结果显示:CXCR2-SiRNAs能显著抑制肝癌细胞的增殖(P<0.05);Transwell小室实验显示,试验组细胞的侵袭能力显著低于对照组[36±5 vs526±9个/高倍镜(×200)](P<0.01)。结论:沉默CXCR2基因体外可有效抑制肝癌细胞的增殖和转移。Objective To investigate the inhibitory effect of antisense CXCR2 on proliferation and invasion of Hepatocellular carcinoma in vitro. Methods The expression of CXCR2mRNA and protein of normal hepatic cell, hepatoma carcinoma cell and the hepatoma carcinoma cell transfected by antisense CXCR2 were detected respectively by RT-PCR and Western blot .The proliferation of HepG2 cells transfected by antisense CXCR2 was measured by CCK- 8; Invasion ability was measured by Transwe11 chamber assay in vitro. Results The expression levels of CXCR2 mRNA and protein in hepG2 cells and normal liver ceils were 1.69 ± 0.22, 0.63± 0.31, 1.93± 0.25 and 0.84 ±0.29 respectively.Compared with normal liver cells.the expression levels of CXCR2 mRNA and protein in HCC cells were significantly higher （P 〈 0.05）. After transfected with CXCR2-SiRNAs, the expression of CXCR2mRNA and protein descended. CXCR2-SiRNAs could significantly inhibit the proliferation of HCC cells （P 〈 0.05 ）. The invasion ability of the trails group was remarkably lower than control group [36±5 vs 526± 9/high power field （× 200）1 （P 〈 0.01）. Conclusion Silencing of CXCR2 gene with siRNA can effectively inhibit the proliferation and invasion of Hepatocellular carcinoma ceils in vitro.

关 键 词：原发性肝癌 CXCR2 反义核甘酸 

分 类 号：R735.7[医药卫生—肿瘤]