白细胞介素-17G197A多态性及其蛋白表达与胃癌的关系  

Association of interleukin-17 G197A gene polymorphism and interleukin-17 expression with gastric cancer

作　　者：吴小琴[1] 曾志荣[2] 许丽霞[3] 于君[3] 曹清华[4] 薛玲[4] 郝元涛[5] 陈旻湖[2] 胡品津[2] 

机构地区：[1]广州医科大学附属第二医院,510260 [2]中山大学附属第一医院消化科,广州市510080 [3]香港中文大学内科及药物治疗学系 [4]中山大学附属第一医院病理科,广州市510080 [5]中山大学公共卫生学院统计教研室,广州市510080

出　　处：《实用医学杂志》2014年第2期246-249,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81201931);广东省自然科学基金(编号:10151008901000223);广州医科大学博士启动项目(编号:2010C06)

摘　　要：目的:分析IL-17 G197A多态性与我国广东地区胃癌遗传易感性的关系,以及IL-17蛋白在胃癌组织中的表达。方法:采用PCR-RFLP法分析1 010例胃癌患者及800例健康对照IL-17 197位点多态性;用Kaplan-Meier生存曲线分析各基因型对患者预后的影响。采用免疫组化法研究54例胃癌患者肿瘤组织和16例非癌胃黏膜组织中IL-17蛋白的表达情况。结果:与野生型GG基因型相比,IL-17 197 AG及AA基因型对胃癌发病风险无显著影响,P值分别为0.098及0.436。IL-17 G197A不同基因型患者之间生存率差异无显著性(P=0.130)。而免疫组化结果则显示IL-17在肿瘤组织中表达显著高于对照的非癌胃黏膜组织(Z=-2.693,P=0.007)。结论:IL-17 G197A多态性与胃癌易感性无关,亦不是影响胃癌患者预后的危险因素,但其蛋白表达水平在胃癌组织中显著提高,提示其可能与胃癌发病有关。Objective To investigate the IL-17 G197A polymorphism in patients with gastric cancer in Guangdoug （low prevalence region） and to investigate IL-17 expression in situ tumors of patients with gastric cancer. Methods One thousand and ten unrelated patients with gastric cancer and 800 age and gender-matched healthy controls were enrolled in this study. Genomic DNA was extracted and used for IL-17 G197A polymorphism analysis by polymerase chain reaction-restriction fragment length polymorphism technique. Kaplan-Meier analysis was used to evaluate the association between IL-17 G197A polymorphisms and survival. Expression of IL-17 in the gastric cancer tissue of 54 gastric cancer patients and in 16 non-tumor gastric mucosa tissues was determined by immunohistochemistry assay. Results The IL-17 197 AG and AA genotypes were not associated with gastric cancer compared with the IL-17 197 GG genotype （P = 0.098 for AG; P = 0.436 for AA）. No significant relationship was observed between IL-17 G197A polymorphism and the survival of GC patients （P 〉 0.05）. However, the immunohistochemistry analysis showed that IL-17 expression was increased significantly in tumor tissue of GC patients （Z = -2.693, P = 0.007）. Conclusion The IL-17 G197A polymorphism was not associated with the susceptibility of gastric cancer and the survival of GC patients. However, the up-regulation of IL-17 in GC tissues may be associated with gastric cancer pathogenesis.

关 键 词：IL-17 基因多态性 生存率 蛋白表达 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]