油茶粕产枯草芽孢杆菌发酵条件优化  

Optimization of Fermentation Conditions for Bacillus Subtilis Production with Camellia Meal

作　　者：黎继烈[1] 胡铁[2] 崔培梧[3] 丁丽霞[1] 钟海雁[1] 李忠海[1] 

机构地区：[1]中南林业科技大学经济林培育与保护省部共建教育部重点实验室,长沙410004 [2]广州航海学院,广州410208 [3]湖南中医药大学药学院,长沙410208

出　　处：《中国粮油学报》2014年第1期52-56,60,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家林业局948计划(2011-4-17);"十一五"国家科技支撑(2009BADB1B10)

摘　　要：以油茶粕为试验原料,通过摇瓶发酵优化产枯草芽孢杆菌的工艺条件。首先通过单因素试验探讨接种量、初始pH、摇瓶装液量、摇床转速、发酵温度和时间对产枯草芽孢杆菌活菌数的影响,在此基础上选择发酵温度、摇床转速、发酵时间3个主要影响因素进行Box-Behnken设计,得到了枯草芽孢杆菌发酵最优工艺条件。结果显示:500 mL摇瓶装培养基120 mL、发酵初始pH 6.8、接种9 mL菌悬液、摇床转速180r/min、发酵温度37℃、发酵时间37 h,发酵液中活菌数可达2.04×1010CFU/mL。By camellia meal as experimental material, the fermentation conditions for Bacillus subtilis production have been optimized on shaking -flask level. Firstly, monofactorial experiments were applied to analyze the effects of inoculation amount, initial pH, amount of medium per flask, table concentrator rotation speed, fermentation temperature and fermentation period on Bacillus subtilis production. Based on the results of monofactorial experiments, fermentation temperature, table concentrator rotation speed and fermentation period were selected to be the significant influence fac- tors. Later optimization experiments were taken on these three factors through Box - Behnken design, and the optimum fermentation conditions for producing Bacillus subtilis had been obtained. The results showed that：on the conditions of 120 mL medium per 500 mL flask,initial pH 6.8,inoculation amount 9 mL,table concentrator rotation speed 180 r/min, fermentation temperature 37℃ ,the living bacteria number in fermentation broth could reach 2.04×1010 CFU/mL.

关 键 词：油茶粕 发酵 枯草芽孢杆菌 

分 类 号：S816.3[农业科学—饲料科学]