经手术切除甲状腺和嗅球的大鼠有可能成为一种难治性抑郁症的实验动物模型  

Thyroidectomized-olfactory bulbectomized rat may be a potential animal model of treatment-resistant depression with hypothyroidism

作　　者：郭灵[1] 莫飞嵘[1] 磨洁琳[1] 

机构地区：[1]广西医科大学人体解剖学教研室,广西南宁530021

出　　处：《大众科技》2013年第12期124-127,共4页Popular Science & Technology

基　　金：广西自然科学基金项目(桂科自0542069);广西高校自然科学基金项目(201012MS035)

摘　　要：目的:探讨建立伴有甲状腺功能低下的难治性抑郁症实验动物模型的可行性。方法:将30只雄性SD大鼠随机分成3组,每组10只:模型组、假手术组、正常对照组。模型组大鼠首先接受甲状腺切除术,7天后再次接受双侧嗅球切除术;对假手术组大鼠除了不切除甲状腺与双侧嗅球以外,其余操作均与模型组的完全相同;对正常对照组大鼠则不进行任何处理。在最终手术后的第2周,采用旷场试验、强迫游泳试验与Morris水迷宫测试各组大鼠的行为变化。结果:与正常对照组或假手术组大鼠相比较,模型组大鼠长生相对停滞、体重明显减轻,并出现焦虑样和抑郁样行为,空间学习记忆能力明显下降。模型组大鼠的这些指标与正常对照组或假手术组大鼠的差异均有统计学意义(P<0.01),但正常对照组与假手术组之间的差异则无统计学意义(P>0.05)。结论:本研究全程应用两种外科手术建立的大鼠模型在原因与行为变化方面,可较好地模拟出伴有甲状腺功能低下的难治性抑郁症的临床特征,故其可能有潜力成为这类难治性抑郁症激越亚型的一种实验动物模型,也为后续开展联合药物治疗与揭示相关药理学机制的研究打下了一个平台。Objective： To examine whether surgical paradigm （TX＋OBX） of thyroidectomy （TX） and olfactory bulbectomy （OBX） performed on rats potentially becomes an animal model of treatment-resistant depression with hypothyroidism. Methods： Thirty Sprague-Dawley adult male rats were randomly divided into three groups with ten rats in each group：model, sham and intact. Both TX and OBX were subsequently performed on rats in model group. After two weeks of OBX operation, open field test, forced swimming test and Morris water maze were used to measure the behavioral changes shown by rats in the three groups. Results： Rats in model group had a lower body weight with a retardation of body growth, and displayed an anxiety-like or agitated depression-like behavior as well as poor ability of spatial learning and memory. These parameter changes in model group rats were statistically different from those in intact group rats or in sham group rats（P0.05）. Conclusion based on the above data, the pattern of TX＋OBX rats largely mimics the etiology and the symptoms of treatment-resistant depression with hypothyroidism , and therefore it potentially becomes a possible animal model of agitated subtype in treatment-resistant depression with hypothyroidism, and may be used as a platform for subsequent intervention of combined thyroid hormone and antidepressant drugs and for exploring relative pharmacological mechanisms of antidepressant therapy.

关 键 词：甲状腺切除术 嗅球切除术 难治性抑郁症 甲状腺功能低下 实验动物模型 

分 类 号：R749.42[医药卫生—神经病学与精神病学]