何首乌不同溶剂提取液的体外抑菌活性研究  

In vitro Antimicrobial Activity of Different Solvent Extracts from Polygonum multiflorum

作　　者：潘小翠[1] 边才苗[1] 管铭[1] 

机构地区：[1]台州学院生命科学学院,浙江台州318000

出　　处：《湖北农业科学》2013年第18期4390-4393,共4页Hubei Agricultural Sciences

基　　金：台州学院青年基金项目(2013QN15)

摘　　要：采用60%丙酮、70%乙醇、沸水3种溶剂制备何首乌(Polygonum multiflorum Thumb)块根粉末提取液,以金黄色葡萄球菌(Staphylococcus aureus)、绿脓杆菌(Pseudomonas aeruginosa)、肺炎克氏菌(Klebsiella peneumoniae)、大肠杆菌(Escherichia coli)为供试菌株,通过滤纸片琼脂扩散法测定提取液的抑菌圈大小,以平板二倍稀释法测定最低抑菌浓度(MIC),以试管二倍稀释法测定最低杀菌浓度(MBC)。结果表明,何首乌3种溶剂提取液对供试菌株均有明显的抑制作用,其中60%丙酮提取液和70%乙醇提取液抑菌效果优于沸水提取液,且对金黄色葡萄球菌的抑制作用最强,丙酮提取液和乙醇提取液的抑菌、杀菌效果随浓度的降低而下降。Polygonum multiflorum root tuber was extracted with 60% acetone,70% ethanol and boiled water,Staphylococcus aureus,Echerichia coli,Pseudomonas aeruginosa and Klebsiella peneumoniae were used as the tested strains.The diameters of inhibition zone were determined by agar diffusion method using filter paper.The minimum inhibitory concentration（MIC） was determined by agar dilution method and minimum bactericidal concentration（MBC） was determined by tube dilution method.The results showed that three extracts of P.multiflorum all had significant inhibition on the tested strains,the inhibitory effect of acetone and ethanol extracts was higher than that of water extracts,and the inhibition was the strongest on Staphylococcus aureu.The antimicrobial activity of acetone and ethanol extracts decreased with the reduction of the concentration.

关 键 词：何首乌 抑菌活性 体外测定 

分 类 号：R284.2[医药卫生—中药学]