姜的脱毒方法比较与繁殖生根同步化培养技术研究  

Comparison of Virus-free Methods and Study on Propagation,Rooting Synchronization Culture Technique in Zingiber officinale

作　　者：韦坤华[1,2] 李林轩[1] 缪剑华[1] 高山林[2] 

机构地区：[1]广西药用植物园/广西药用资源保护与遗传改良重点实验室,南宁530023 [2]中国药科大学遗传育种教研室,南京211198

出　　处：《湖北农业科学》2013年第18期4522-4525,共4页Hubei Agricultural Sciences

摘　　要：考察了茎尖大小、热处理催芽结合茎尖培养以及催芽后高温热处理结合茎尖培养等对姜（Zingiber officinale Rosc.）茎尖培养脱毒效果影响,并进行了脱毒姜试管苗的繁殖生根同步化培养基筛选。结果表明,0.3 mm的茎尖能够获得较为理想的成活率、成苗率,并且具有较好的脱毒效果;热处理结合茎尖培养能够提高脱毒效果,且催芽后高温短时间热处理的脱毒效果优于热处理直接催芽;确定脱毒姜繁殖生根同步化培养的最佳培养基为MS＋2.50 mg/L 6-BA＋0.4 mg/L NAA＋0.2~0.3 mg/L ABT,在此培养基上生长的试管苗长势旺盛,根系发达,移栽成活率高。Stem tip meristem culture was the most effective virus-free method of Zingiber officinale.The virus-free effects of meristem culture combined with different heat treatment methods were compared,and also screened the propagation and rooting synchronization medium for virus-free plantlets of Z.officinale.The results showed that the suitable size for meristem culture was 0.3 mm,the best combined method of virus-free was high temperature treatment after sprouting combined with meristem culture,and the best propagation and rooting synchronization medium for Z.officinale was MS＋2.50 mg / L 6-BA＋0.4 mg / L NAA＋0.2~0.3 mg / L ABT.

关 键 词：姜(Zingiber officinale ROSC ) 脱毒方法 分生组织培养 同步化培养 

分 类 号：S632.5[农业科学—蔬菜学]