弹力纤维染色在评估肺癌胸膜侵犯中的意义  

Clinical significance of elastic fibers staining in assessing the pleural invasion of lung cancer

作　　者：赵兰香[1] 余科科[1] 邵晋晨[1] 朱蕾[1] 韩非[1] 刘尽国[1] 丁闻杰 叶敏[1] 秦钢[1] 张杰[1] 

机构地区：[1]上海交通大学附属胸科医院病理科,上海200030

出　　处：《临床与实验病理学杂志》2014年第1期22-24,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨胸膜弹力纤维(elastic fibers,EFs)染色在肺癌TNM分期应用中的价值。方法随机选取手术切除的肺癌标本200例,肉眼观察癌组织均紧贴胸膜。石蜡切片,行HE及EFs染色。按照WHO(2004)肺癌分类标准,对200例标本进行组织学类型分类、观察并分析HE及EFs染色结果。结果光镜下对200例标本进行组织学分类,其中162例为腺癌、29例为鳞癌、4例为腺鳞癌、5例为大细胞癌;其中肿瘤细胞未突破胸膜弹力层92例,突破胸膜弹力层108例(含破脏层胸膜20例);HE染色28例无法判断或判断不清,最终经EFs染色得出结论。结论应用胸膜EFs染色,能直观地观察肿瘤细胞是否到达或突破胸膜层,有助于准确评估肺癌的胸膜侵犯和TNM分期。Purpose To study the value of pleural elastic fibers （elastic fibers, EFs） staining in the TNM staging of lung cancer. Methods 200 cases of surgical resected lung cancer with closing to the pleura on gross were randomly selected. The 200 cases were subclassificated according to 2004 WHO classification criteria under the microscope. Then they were performed with paraffin sections, HE staining and EFs staining. Results 162 cases were adenocarcinoma, 29 cases were squamous cell carcinoma, 4 cases were adeno- sqnamous carcinoma, and 5 cases were large cell carcinoma. 92 cases showed that the tumor cells didn＇ t break the elastic layer of the pleura, 108 cases broke the pleura including 20 cases which broke the visceral pleura. In the meantime, there were 28 cases which didn＇ t judge by HE staining, and then they were judged by EFs staining. Conclusion EFs staining on pleura can be used to observe whether the tumor cells reach or break through the pleural layers clearly and visually. So it can help to determine the TNM staging of lung cancer accurately.

关 键 词：肺肿瘤 弹力纤维 组织化学染色 

分 类 号：R361.2[医药卫生—病理学]