Rab25基因对乳腺癌裸鼠移植瘤生长的影响  

Effects of Rab25 gene on the growth of implanted tumor of breast cancer in nude mice

作　　者：张小彬[1] 陆云飞[1] 沈桂鑫[1] 吴非[1] 

机构地区：[1]广西医科大学第一附属医院,南宁市530021

出　　处：《实用医学杂志》2014年第3期361-364,共4页The Journal of Practical Medicine

基　　金：广西医疗卫生重点课题(编号:重200635)

摘　　要：目的:建立一系列Rab25表达水平不同的乳腺癌细胞裸鼠移植瘤模型,观察不同水平Rab25表达对乳腺癌裸鼠移植瘤的影响。方法:通过分别上调及沉默Rab25基因在乳腺癌细胞中的表达,建立Rab25表达水平不同的乳腺癌细胞的裸鼠移植瘤模型,测量肿瘤体积,绘制肿瘤生长曲线,检测肿瘤组织中Rab25表达差异。结果:Rab25转染组较干扰组、原株细胞组及空质粒组对裸鼠细胞致瘤能力明显增强。转染组裸鼠瘤体中Rab25 mRNA表达明显增强,而干扰组瘤体Rab25 mRNA表达明显减弱。结论:Rab25为促癌基因,其高表达可直接增强乳腺癌细胞的增殖侵袭能力,而RNA干扰技术有可能为乳腺癌的肿瘤靶向和基因治疗提供新的思路。Objective To establish nude mice models with implanted tumor of breast cancer cells with different expressing levels of Rab25 gene, and to observe the effects of Rab25 gene on the growth of implanted tumor. Methods Nude mice models with implanted tumor of breast cancer cells with different expressing levels of Rab25 gene were constructed through up-regulating or silencing the expression of Rab25 gene in breast cancer cells. The size of implanted tumor was measured, the growth curve of tumor was portrayed, and the expression of Rab25 gene in tumor tissues was detected. Results The tumorigenic effect on nude mice was the strongest and the highest in Rub25 up-regulating group, as compared to those in Rub25 interference group, original cells group, and Rub25 empty plasmid group. The expression of Rab25 mRNA in interference group was significantly reduced. Conclusions Rub25 is a cancer-promoting gene, its high expression could directly enhance the proliferation of breast cancer cells. RNA interference technology had the potential to provide new ideas for tumor targeting and gene therapy for breast cancer.

关 键 词：乳腺肿瘤 Rab25基因 裸鼠移植瘤 

分 类 号：R737.9[医药卫生—肿瘤]