化疗对噬菌体展示肽法检测大肠癌自身抗体效果的影响  

Effect of chemotherapy on detection of colorectal cancer autoantibodies by phage display peptide method

作　　者：刘岩[1] 常文军[1] 曹广文[1] 

机构地区：[1]第二军医大学热带医学与公共卫生学系流行病学教研室,上海市医学生物防护重点实验室,上海200433

出　　处：《第二军医大学学报》2014年第1期85-88,共4页Academic Journal of Second Military Medical University

基　　金：上海市登山计划重大课题(06DZ19503)~~

摘　　要：目的探讨化疗对噬菌体展示肽法检测大肠癌自身抗体效果的影响。方法选取前期研究筛选出的5个(95号,149号,174号,396号,1009号)能高效区分大肠癌患者血清和健康对照者血清的大肠癌相关噬菌体克隆作为检测噬菌体,采用酶联免疫吸附试验(ELISA)检测该5个检测噬菌体克隆与20例化疗大肠癌患者、40例非化疗大肠癌患者及40例健康对照者血清的反应性。结果 95号检测噬菌体与大肠癌化疗患者血清反应性低于与非化疗大肠癌患者血清反应性(P<0.05),该噬菌体在健康对照组及化疗大肠癌患者组的血清反应性差异无统计学意义(P=0.074)。149号、174号、396号、1009号噬菌体在健康对照组及化疗大肠癌患者组的血清反应性差异有统计学意义(P均<0.01)。结论化疗对149号、174号、396号、1009号噬菌体的检测效果影响不明显,而95号噬菌体对肿瘤自身抗体的检测能力受化疗影响明显。Objective To evaluate the effect of chemotherapy on detection of colorectal cancer autoantibodies by phage display peptide method. Methods The five-phage peptide clones （No. 95, No. 149, No. 174, No. 396, and No. 1009） with high discriminatory ability of colorectal cancer patients were selected as the study subjects. We compared the reactivity of autoantibodies against each of the five-phage peptide clones among 20 colorectal cancer patients receiving chemotherapy, 40 colorectal cancer patients receiving no chemotherapy, and 40 healthy controls by enzyme linked immunosorbent assay （ELISA）. Results The seroreactivity of No. 95 clone was significantly lower in the patients with chemotherapy than those without chemotherapy （P^0.05）, and was similar between normal control group and patients with chemotherapy （P=0. 074） . For the seroreactivity of the other four clones （No. 149, No. 174, No. 396, No. 1009）, there were significant differences between patients with chemotherapy and normal controls （all P〈0.01）. Conclusion The detection ability of No. 95 clones for colorectal cancer autoantibodies is greatly influenced by chemotherapy, while chemotherapy shows no notable influence on the reactivity of other four-phage peptide clones.

关 键 词：结直肠肿瘤 肽库 药物疗法 自身抗体 

分 类 号：R735.34[医药卫生—肿瘤]