legumain在胃癌中的表达及其临床意义  

Prognostic significance of legumain overexpression in gastric cancer patients

作　　者：冯倩[1] 刘杞[1] 向荣[1] 孙航[1] 刘燕倩 胡怀东[1] 

机构地区：[1]重庆医科大学附属第二医院病毒性肝炎研究所,国家教育部感染性疾病分子生物学重点实验室,重庆400010

出　　处：《第三军医大学学报》2014年第3期232-235,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30572116)~~

摘　　要：目的探讨legumain在胃癌中的表达以及临床意义。方法采用免疫组织化学染色方法检测胃腺癌组织芯片上50例胃癌组织及2007年9月至2011年6月期间重庆医科大学附属第二医院普通外科收治的210例胃癌患者(其中男性140例,女性70例,平均年龄65岁)经临床手术切除的胃癌组织中legumain的表达,分析其与多项临床指标间的相关性,运用单因素和多因素生存分析研究legumain表达以及各项临床指标与预后的关系。结果芯片上50例胃癌组织中,29例(58%)高表达legumain,而在50例相应正常组织中只有16例(32%)高表达legumain,胃癌组织中legumain表达较相应正常组织中高(P<0.05);在210例临床手术切除胃癌组织切片中,124例(59%)高表达legumain;legumain与多项临床指标间具有一定程度的相关性;生存分析结果显示legumain高表达(P<0.01)、MMP-9高水平(P<0.05)、肿瘤远处转移(P<0.01)以及肿瘤低分化(P<0.01)是影响胃癌预后的因素。结论 legumain在胃癌组织中高表达,高表达legumain的胃癌患者其生存率明显降低,legumain高表达是影响胃癌临床预后的独立危险因素。Objective To study the expression of legumain in gastric cancer patients and its prognostic significance. Methods Legumain expression was tested with immunohistochemical staining in 50 cases of gastric cancer tissues and 50 corresponding normal tissues on tissue chips and in 210 cases of gastric cancer patients （including 140 male and 70 female with mean age of 65 years, which were treated in the Second Affiliated Hospital of Chongqing Medical University from September 2007 to June 2011 ）. The correlation between legumain expression and various clinical parameters was evaluated. Both the univariate and multivariate survival analysis were performed to evaluate the prognostic significance of legumain expression and all the clinical parameters. Results On the tissue chips, 29 （58%） of 50 gastric cancer tissues and only 16 （32%） of 50 corresponding normal tissues showed legumain overexpression, with statistical difference （P 〈 0. 05）. Legumain overexpression was also observed in 124 （59%） of 210 gastric cancer patients. High expression of legumain was correlated with various clinical parameters. Patients with legumain overexpression （P 〈 0.01 ）, high level of MMP-9 （ P 〈 0.05 ）, distant metastasis （ P 〈 0.01 ） and poor tumor differentiation （P 〈 0.01 ） resulted in bad prognosis. Conclusion Legumain is overexpressed in gastric cancer tissues compared with corresponding normal tissues. Gastric cancer patients with higher expression of legumain have a lower survival rate. Legumain overexpression is an independent risk factor for clinical prognosis of gastric cancer patients. Legumain may affect the survival rate of gastric cancer patients via increase the level of MMP-9 and tumor metastasis.

关 键 词：LEGUMAIN 胃癌 MMP-9 远处转移 预后 

分 类 号：R345[医药卫生—基础医学] R730.23