STZ单次侧脑室注射诱导的大鼠AD空间认知能力损伤与早期病理改变  

Streptozotocin cerebralventricular injection induced early stage AD-like spatial cognitive impairment and brain pathological changes

作　　者：赵娟[1] 刘珂[1] 李琳[1] 

机构地区：[1]山西医科大学省教育部共建细胞生理学重点实验室,太原030001

出　　处：《神经解剖学杂志》2014年第1期93-97,共5页Chinese Journal of Neuroanatomy

摘　　要：目的:探索链脲霉素(STZ)单次侧脑室注射诱导的大鼠AD样空间认知能力损伤与早期病理改变。方法:SD大鼠侧脑室单次注射STZ(3 mg/kg),恢复2周后利用Morris水迷宫测定其空间学习、记忆能力,用免疫组织化学研究其β淀粉样蛋白(Aβ)和tau蛋白的磷酸化水平;用Western Blot测定淀粉样前体蛋白(APP)的表达。结果:单次STZ侧脑室注射可诱导大鼠学习、记忆能力损伤,tau蛋白异常磷酸化;但未能诱导Aβ的异常聚集及其前体蛋白APP的异常表达。结论:结果提示,脑内胰岛素信号异常的状态可能诱导动物空间认知功能的损害和及脑内tau蛋白的异常磷酸化。Objective： To explore streptozotocin cerebralventricular injection（ICV）-induced early stage AD-like spatial cognitive impairment and brain pathological changes. Methods：We administrated the SD rats with 3 mg/kg STZ ICV then used Morris water maze （MWM） to explore the spatial learning and memory ability, to detect the aggregation of Aβ and tau phosphorylational level by immunohistochemistry, and the expression of amyloid-beta precursor protein （APP） by Western Blot. Results： STZ ICV rats performed an impaired spatial learning and memory ability and the hyperhosphoryla- tion of tau protein, but it did not show significant Aβ aggregation with the expression changes of APP. Conclusion： Our study suggests that insulin signalling failure could induce spatial cognitive impairment and tau abnormal phosphorylation.

关 键 词：链脲霉素 Alzheimer病 Moms水迷宫 tau beta-淀粉样蛋 淀粉样前体蛋白 

分 类 号：R749.16[医药卫生—神经病学与精神病学]