高效液相色谱/二极管阵列检测器同时测定辣椒及其制品中11种脂溶性着色剂  

Simultaneous Determination of 11 Fat-soluble Colorants in Foodstuffs by HPLC/DAD

作　　者：马育松[1] 李玮[1] 艾连峰[1] 郭春海[1] 陈瑞春[1] 

机构地区：[1]河北出入境检验检疫局,河北石家庄050051

出　　处：《分析测试学报》2014年第1期93-97,共5页Journal of Instrumental Analysis

基　　金：国家认监委检验检疫行业标准项目(2009B541)

摘　　要：建立了一种简单、快速同时测定辣椒及其制品中苏丹橙G、甲基黄、对位红、柑桔红2号、苏丹红G、苏丹Ⅰ、苏丹Ⅱ、苏丹Ⅲ、苏丹红7B、苏丹红B、苏丹Ⅳ11种脂溶性着色剂的分析方法.样品经乙酸乙酯提取后采用凝胶渗透色谱净化,用配有二极管阵列检测器的高效液相色谱法测定,外标法定量.结果表明,11种着色剂在20～1 000 μg/L范围呈良好线性,线性系数均大于0.998.在10、20、40 μg/kg 3个加标水平下的平均回收率为75.8％～ 90.8％,相对标准偏差为3.0％～7.8％,11种着色剂的定量下限为10μg/kg.该方法稳定、可靠,适用于辣椒和辣椒酱中11种着色剂的测定.A simple and rapid method was developed for the simultaneous determination of 11 fat-soluble colorants,including Sudan Orange G,Dimethyl Yellow,Para Red,Citrus Red 2,Sudan Red G,Sudan Ⅰ,SudanⅡ,Sudan Ⅲ,Sudan Red 7B,Sudan Red B and Sudan Ⅳ in chilies.The sample was extracted with ethyl acetate and then purified by gel permeation chromatography （GPC）.The targeted compounds were detected by HPLC/DAD detector and quantified by the external standard method.The calibration curves of 11 colorants were linear in the range of 20-1 000 μg/L with correlation coefficients more than 0.998.The average recoveries of 11 colorants at three spiked levels of 10,20,40 μg/kg were between 75.8％ and 90.8％,with relative standard deviations of 3.0％-7.8％.The quantitation limits（S/N≥ 10）were all 10 μg/kg.The method is convenient and accurate,and could be applied in the determination of 11 colorants in chilies and chili sauce.

关 键 词：凝胶渗透色谱 高效液相色谱 二极管阵列检测法 着色剂 辣椒 

分 类 号：O657.72[理学—分析化学] TS193.2[理学—化学]