高效液相色谱法测定骨刺丸中欧前胡素含量  

Determination of Imperatorin Content in Guci Pill by HPLC

作　　者：李学红 巩长芹 周利章 

机构地区：[1]山东省临沂市药品检验所,山东临沂276001

出　　处：《中国药业》2014年第2期30-31,共2页China Pharmaceuticals

摘　　要：目的建立骨刺丸中欧前胡素的含量测定方法。方法采用十八烷基硅烷键合硅肢柱，检测波长为300 nm,流动相为曱醇-水（60 ： 40），柱温为35 ℃,流速为1.0mL/min。结果欧前胡素进样量在0. 028 4 - 0. 113 7叫范围内与峰面积呈良好线性关系（r = 0. 999 8），平均回收率97.01% = 0.99% （ra = 6）。结论该方法操作简便、准确性好。Objective To establish a method for determination of imperatorin content in Guci Pill. Methods The octadecyl silane bond- ed silica gel column was adopted with methanol - water （60 ：40） as the mobile phase at a flow rate of 1. 0 mL/min,the temperature was 35 t and the detection wavelength was 300 nm. Results The linear range of imperatorin was 0.028 4-0. 113 7｜xg （r = 0.999 8） and the average recovery rate of imperatorin was 97. 01% , RSD = 0.99% （ n = 6）. Conclusion This method is simple to operate and good in accuracy, and can be used as the determination method of the imperatorin content of Guci Pill.

关 键 词：骨刺丸 欧前胡素 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]