HPLC法评价河南不同产地野葛质量的实验研究  

Quality Evaluation of Radix Puerariae Lobatae in Different Origin of Henan Province by HPLC

作　　者：鲁艳清[1] 代丽萍[1] 王娟娟[1] 冯璐洁 

机构地区：[1]河南中医学院,河南郑州450046

出　　处：《中国药物评价》2013年第6期331-333,共3页Chinese Journal of Drug Evaluation

基　　金：河南省教育厅重点研究资助(12A360001);河南中医学院博士基金资助(BSJJ2010-02)

摘　　要：目的:以野葛中主要异黄酮的含量为指标探讨河南野葛质量评价方法。方法:采用HPLC法测定不同产地野葛中葛根素大豆苷、大豆苷元的含量,ODSHYPERSIL色谱柱(4.6 mm×250 mm,5μm);乙腈-水梯度洗脱;柱温30℃;流速0.8 mL/min;检测波长250 nm。结果:葛根素、大豆苷、大豆苷元分别在0.1632～1.3056、0.0700～0.5632、0.004796～0.03837μg内线性关系良好;平均回收率分别为101.51%,98.08%,101.04%;相对标准偏差(RSD)分别为1.11%、1.35%、2.12%;结论:本实验建立的方法简便、准确、重复性好,可以用于河南不同产地野葛的质量评价。Objective： To evaluate the quality of radix puerariae lobatae in different origin of Henan province based on the contents of main isoflavones in it. Methods： Establish a method of HPLC for quantitative determination of puerarin, daidzin and daidzein in radix pu- erariae lobatae. The procedure of HPLC was performed on the chromatographic column of ODS HYPERSIL（4. 6 mm × 250 mm,5 μm）, and the mobile phase in the gradient elution program was acetonitrile-water. The column temperature was 30 ℃, the flow rate was 0. 8 mL/min, and the detection wavelength was 250 nm. Results：The three compounds showed good linear relationship within the range of 0. 1632 - 1. 3056,0. 0700 - 0. 5632 and 0. 004796 - 0. 03837 μg. The average recovery were 101.51% （ RSD = 1.11% ）, 98.08% （RSD = 1.35% ） , 101.04% （RSD = 2. 12% ） respectively. Conclusion： The method is easy and accurate with higher repeatability, which can be used in the quality control of radix puerariae lobatae.

关 键 词：野葛 葛根素 大豆苷 大豆苷元 质量控制 

分 类 号：R282.5[医药卫生—中药学] O657.72[医药卫生—中医学]