改善玉米秸秆酶水解糖化得率的碱性亚硫酸盐法预处理工艺的优化  

Optimization of alkaline sulfite pretreatment of corn stover for improving enzymatic hydrolysis saccharification yield

作　　者：刘欢[1,2] 庞博[1,2] 李海明[1] 牛梅红[1] 王海松[2] 

机构地区：[1]大连工业大学轻工与化学工程学院,辽宁大连116034 [2]中国科学院生物燃料重点实验室(青岛生物能源与过程研究所),山东青岛266101

出　　处：《中华纸业》2014年第2期10-14,共5页China Pulp & Paper Industry

基　　金：国家自然科学基金项目资助(编号:21206184;31370582)

摘　　要：对碱性亚硫酸钠法预处理玉米秸秆的工艺进行了优化,确定了最佳的预处理条件为用碱量12%,液固比为6∶1,最高温度140℃,保温时间20min。在该预处理条件下的葡聚糖的酶水解效率为85.38%,木聚糖的酶水解效率为70.36%,总糖得率为74.73%,相比相同总碱量氢氧化钠预处理秸秆酶水解总糖得率67.67%,提高10.43%。此外,在此最佳预处理条件下处理的玉米秸秆,使用PFI继续打浆1500转后,葡聚糖的酶水解效率为89.74%,木聚糖的酶水解效率为74.06%,总糖得率为78.58%,相比相同总碱量氢氧化钠预处理秸秆后再PFI处理1500转的总糖得率68.90%,提高14.05%。In this study, alkali sulfite pretreatment of corn stover was optimized. The optimum pretreatment conditions were obtained, as follows： the total titratable alkali of 12%, liquid ratio of 6：1, temperature of 140℃ and the holding time of 20min. With the pretreatment, subsequent enzymatic hydrolysis rates of glucan and xylan were 85.38% and 70.36%, respectively, and the total sugar yield was 74.73%. Compared with sodium hydroxide pretreatment at the same amount of total titratable alkali, the total sugar yield improvement rate was 10.43%. Additionally, the corn stover pretreated under the optimum pretreatment conditions was beaten by PFI for 1500 rotations. After beating, enzymatic hydrolysis rates of glucan and xylan were 89.74% and 74.06%, respectively, and the total sugar yield was 78.58%. Compared with 1500 rotations of PFI beating after sodium hydroxide pretreatment at the same amount of total titratable alkali, the total sugar yield improvement rate was 14.05%.

关 键 词：玉米秸秆 碱性亚硫酸钠法预处理 酶水解 总糖得率 

分 类 号：TS721.3[轻工技术与工程—制浆造纸工程] TS743.11