白藜芦醇对小鼠酒精性肝损伤的抑制作用  

Inhibitive effects of resveratrol on alcohol liver injury in mice

作　　者：周晓云[1] 崔晓明 崔国金[1] 蔡庆艳[1] 韩淑英[1] 

机构地区：[1]河北联合大学基础医学院,河北唐山063000 [2]内蒙古自治区科左后旗人民医院

出　　处：《河北联合大学学报（医学版）》2014年第1期3-5,共3页Journal of North China Coal Medical College

摘　　要：①目的观察白藜芦醇对乙醇所致酒精性肝损伤的抑制作用,探讨可能机制。②方法用50%乙醇灌胃造成肝损伤模型,同时用高(0.08g/kg)、中(0.04g/kg)、低(0.02g/kg)剂量白藜芦醇灌胃干预,设正常和模型对照组,连续灌胃10天。检测指标:小鼠一般状态,测定体质量,肝重与肝指数;血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;肝组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;小鼠肝组织病理变化。③结果与正常组比较,模型组小鼠体质量和肝指数降低,血清ALT及AST活性显著升高,肝匀浆MDA含量明显升高,SOD含量降低;与模型组比较,白藜芦醇组小鼠体质量和肝指数有所升高,血清ALT及AST活性显著降低,肝匀浆MDA明显降低,SOD含量升高,并呈明显量效关系;模型组小鼠肝细胞明显气球样变性、坏死和明显炎细胞浸润,白藜芦醇能明显改善肝细胞坏死病变程度。④结论白藜芦醇对酒精所致小鼠肝损伤有一定的抑制作用,对肝细胞起保护作用,其机制可能与抑制脂质过氧化损伤有关。Objetc ive To investigate the inhibitive effects of resveratrol on alcohol liver injury in mice and research the possi -ble mechanism .Methods The rats were randomLy divided into 5 groups , including normal control group ,alcohol-induced hapatic injuried model group, Res 0.08 g /kg group, Res 0.04g /kg group and Res 0.02 g /kg group.The hepatic injuried model was imi-tated by 55%ethanol.Res was administrated continuously for 10 days.Detection index：The mice general state,determination of body weight,liver weight and liver index;Serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST） activity;liver tissue malondialdehyde （ MDA） content and superoxide dismutase （ SOD） activity;pathological changes in liver tissue in mice .Results Compared with the normal control group ,the concentrations of mice＆#39;weight ,liver determination and SOD were significantly reduced and ALT,AST ,MDA were significantly increased in the model group .Meantime,in the Res group,the level of weight ,liver determi-nation and SOD were increased and ALT ,AST,MDA were reduced ,compared with model group ,furthermore,into a clear dose -effect relationship .Model mice liver cells was ballooning degeneration , necrosis and marked infiltration of inflammatory cells , res-veratrol can significantly improve the severity of liver cell necrosis .Conclusion Res have a certain inhibitory effect on alcohol -in-duced liver damage and play a protective role in liver cells ,the mechanism may be related to inhibition of lipid peroxidation damage .

关 键 词：白藜芦醇 小白鼠 酒精性肝损伤 

分 类 号：R575[医药卫生—消化系统]