美洲南瓜(Cucurbit pepo)WMV-2抗性遗传及分子标记研究  

Studies on Inheritance of Resistance to WMV-2 and Molecular Markers of Pumpkin(Cucurbit pepo)

作　　者：王冬杰[1] 葛宇[1] 刘超[1] 李雪[1] 徐文龙[1] 崔崇士[1] 屈淑平[1] 

机构地区：[1]东北农业大学园艺学院,黑龙江哈尔滨150030

出　　处：《河南农业科学》2014年第1期79-83,共5页Journal of Henan Agricultural Sciences

基　　金：公益性行业(农业)科研专项经费项目(201303112);东北农业大学博士启动基金项目(2012RCB30)

摘　　要：以美洲南瓜(Cucurbita pepo)抗病自交系‘0504’和感病自交系‘0223’为亲本杂交获得的157个F2单株为材料,研究南瓜对西瓜花叶病毒2号(WMV-2)抗性的遗传规律和分子标记。对亲本、F1及F2苗期接种WMV-2进行抗病性鉴定,结果表明,美洲南瓜对WMV-2的抗性由单隐性基因控制。采用集团分离分析法(BSA)和SSR技术,筛选出与WMV-2抗性基因连锁的标记CMTm157和CMTm158,遗传距离分别为10.9cM和6.2cM,其可以作为南瓜抗WMV-2育种的辅助选择分子标记。With 157 pumpkin （Cucurbita pepo） F2 plants as material ,which were generated by hybridi-zing disease-resistant inbred line‘0504’with susceptible inbred line‘0223’ ,the genetic regularity and mo-lecular markers of pumpkin resistance to watermelon mosaic virus 2 （WMV-2）were studied .The pa-rents ,F1 and F2 plants were inoculated with WMV-2 at seedling stage for resistance identification ,and the results showed that the pumpkin resistance to WMV-2 was controlled by a single recessive gene .Bulked separation analysis （BSA） and simple sequence repeats （SSR） technology were utilized jointly and two SSR markers linked to WMV-2 resistance genes ,CMTm 157 and CMTm 158 ,were filtered out .The ge-netic distance of the two SSR markers was 10 .9 cM and 6 .2 cM ,respectively ,which can be used for as-sisted selection in pumpkin anti-WMV-2 breeding .

关 键 词：美洲南瓜 西瓜花叶病毒 抗性遗传规律 SSR标记 

分 类 号：S436.42[农业科学—农业昆虫与害虫防治]