美兰抑制变形链球菌产酸的实验研究  

The experimental study on streptococcus mutans acid production inhibition by methylene blue

作　　者：王成龙[1] 李振纲[2] 赵皿[1] 沈世杰[3] 

机构地区：[1]第四军医大学口腔医学院,西安710032 [2]中国人民解放军总医院 [3]军事医学科学院

出　　处：《临床口腔医学杂志》2000年第4期202-203,共2页Journal of Clinical Stomatology

摘　　要：目的　研究美兰对变形链球菌在外源性糖培养条件下产酸的抑制作用 ,探讨美兰作为一种新型防龋制剂的可行性。方法　通过将美兰加入变形链球菌糖培养液的方法 ,以不加美兰者作对照 ,用气相色谱法检测不同培养条件下变形链球菌培养液中产生有机酸的种类和数量。结果　美兰组有机酸的总量为 14.6 6± 5 .42 mmol/L,与对照组比较相差显著 (P<0 .0 1) ;其中以乳酸的量为最高 ,且均未检出丙酸和丁酸。结论　美兰具有抑制变形链球菌产酸的作用WT5”BZ]Objective To study Streptococcus mutans acid production inhibition by methylene blue, and investigate the practicability of methylene blue as new kind of dental caries prevention agent. Methods The kind and quantities of acid produced by Streptococcus mutans in three different culture fluids, NS, 0.5M glucose, 0.5M glucose+0.025%(g/L) methylene blue, were measured with gas chromatography. Results There were significant difference between the total quantities of acid in three different culture fluids, their were respectively glucose+methylene blue: 14.66±5.42mmol/L, NS:26.00±5.73mmol/L, glucose: 42.99±7.86mmol/L. However, the kinds of acid in three ctuture fluids were same, in which lactic acid was the greatest among all acids, and methylacetic acid, butyric acid were not found. Conclusion methylene blue has acid production inhibition to Streptococcus mutans, and it is worth kind of dental caries prevention agent. [WT5”HZ]

关 键 词：美兰 变形链球菌 产酸抑制 龋齿 

分 类 号：R781.101[医药卫生—口腔医学]