靶向肿瘤腺病毒介导IL-24抑制人胃癌细胞增殖  

Suppression of gastric cancer cell growth by tumor-targeted adenovirus mediated IL-24 gene

作　　者：曹亚玲[1] 黄茂涛[1] 黄一[2] 季代金[1] 冯早明[1] 李学成[3] 陈陵[2] 

机构地区：[1]解放军452医院消化内科,成都610021 [2]解放军324医院消化内科 [3]成都军区峨眉疗养院

出　　处：《西南国防医药》2014年第1期9-12,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金资助课题(30900679);重庆市集成示范计划项目(cstc2013jcsf10014);重庆市医学科研计划项目(2011-2-587);四川省卫生厅科研课题(110484)

摘　　要：目的探讨人端粒酶逆转录酶(hTERT)启动子特异驱动IL-24的重组腺病毒(Ad-phTERT-IL-24)对人胃癌SGC-7901细胞增殖及侵袭能力的影响。方法将Ad-phTERT-IL-24感染人胃癌SGC-7901细胞,采用荧光实时定量PCR及Western blot检测IL-24表达;采用CCK-8方法检测细胞生长曲线;采用Transwell小室侵袭实验检测细胞侵袭能力。结果与以PBS代替病毒液处理的SGC-7901细胞相比,感染Ad-phTERT-IL-24的SGC-7901细胞内IL-24表达明显升高(P<0.01),于第5、6 d吸光度明显下降(P<0.05),细胞侵袭能力亦显著降低(P<0.05)。结论 Ad-phTERT-IL-24可有效上调人胃癌SGC-7901细胞IL-24表达,进而抑制细胞增殖及侵袭能力。Objective To discuss the effects of human telomerase reverse transcriptase （hTERT）promoter targeted recombinant adenoviral vector carrying human IL-24 gene（Ad-phTERT-IL-24） on the proliferation and infestation ability of SGC-7901 human gastric cancer ceils. Methods SGC-7901 tumor cells were infected by Ad-phTERT-IL-24. IL-24 expression level was examined by RT-qPCR and Western blot. CCK8 assay was used to detect the cell growth curve. Transwell camber infestation experiment wascarried out to evaluate the invasion capability of SGC-7901 tumor cells. Results The expression of IL-24 in the SGC-7901 cells infected with Ad-phTERT-IL-24 significantly increased compared with the cells treated with PBS instead of virus liquid（ P 〈0.01 ） ,the absorbance decreased significantly since the fifth and sixth day（P 〈0.05 ）. Meanwhile the invasion ability also decreased significantly （P 〈 0. 05 ）. Conclusion Ad-phTERT-IL-24 can effectively up-regulate the IL-24 expression and furthermore suppress the proliferation and invasion ability of human SGC-7901 gastric cancer cells.

关 键 词：端粒酶 逆转录酶 启动子 腺病毒 白细胞介素-24 胃癌 

分 类 号：R739[医药卫生—肿瘤]