酒精性肝病细胞色素P450ⅡE_1基因型分析及其意义  

Genotyping cytochrome P450ⅡE_1 in alcoholic liver diseases and its significance

作　　者：张顺财[1] 刘斯青[1] 刘红春[1] 朱无难[1] 

机构地区：[1]上海医科大学附属中山医院消化科,200032

出　　处：《中华肝脏病杂志》2000年第6期338-339,共2页Chinese Journal of Hepatology

摘　　要：目的：研究细胞色素P450ⅡE1（简称ⅡE1）基因型与酒精性肝病形成之间的关系。 方法：采用外周血白细胞DNA，经PCR及限制性内切酶消化方法，对ⅡE1基因型进行分析。 结果：ⅡE1可分为Ａ、Ｂ、C三型。正常人群以Ａ型为主，Ｂ型次之，Ｃ型罕见。非酒精性肝病的形成与ⅡE1基因型无关，其基因型分布类似正常人群。酒精性肝病与正常人群及其他肝病组对照，Ａ型明显降低，Ｂ型明显增高。酒精相关性肝癌患者的Ａ型也明显减少，而Ｂ型明显增多。结论：ⅡE1基因型在形成酒精性肝病及导致酒精性肝癌中起一定作用。Objective: To study the relationship between genotypes of cytochrome P450ⅡE1 (ⅡE1 ) and development of chronic liver diseases. Methods: Cytochrome P450ⅡE1 was genotyped by PCR and restricted endonucleases digestion of DNA prepared from peripheral white blood cells. Results: ⅡE1 could be divided into three types: A, B and C based on different products digested by two endonucleases. In normal controls predominant genotypes were A and B. No relationship was found between development of non-alcoholic chronic liver diseases and ⅡE1 genotypes. Its genotype distribution was similar to normal controls. Detection of type A in alcoholic liver diseases was significantly decreased and type B was significantly increased (P<0.01). Detection of type A in alcohol-related hepatocellular carcinoma was also decreased and type B increased compared to non-alcohol-related hepatocellular carcinoma. Conclusion: ⅡE1 genotypes play some roles in development of alcoholic liver diseases and alcohol-related hepatocellular carcinoma.

关 键 词：细胞色素P450ⅡE1 酒精性肝病 基因型 

分 类 号：R575[医药卫生—消化系统]