左旋精氨酸对缺血-再灌注损伤肝脏的保护作用  

Protective effect of L-arginine on liver during ischemia-reperfusion injury

作　　者：王万铁[1] 徐正介[1] 林丽娜[2] 王卫[1] 李东[3] 王宗敏[4] 

机构地区：[1]温州医学院病理生理教研室,温州325003 [2]温州医学院附属第一医院麻醉科 [3]温州医学院检验系分析中心,温州325003 [4]温州医学院附属第二医院病理科

出　　处：《中华肝脏病杂志》2000年第6期370-372,共3页Chinese Journal of Hepatology

基　　金：浙江省卫生厅科研基金!(NO.98A087)

摘　　要：目的：探讨左旋精氨酸（L-arginine，L-Arg）对肝缺血－再灌注损伤（hepatic ischemia-reperfusion injury，HIRI）的防治作用及其机理。方法：选择HIRI家兔及肝手术患者，观察一氧化氮水平、丙二醛浓度、血栓素B2和6－酮基－前列腺素F1α含量、血栓素B2/6－酮基－前列腺素F1α比值、ALT活性、肝细胞形态学的变化及L-Arg对上述指标的影响。结果：HIRI期间，NO、6－酮基－前列腺素F1α显著下降，MDA、血栓素B2、血栓素B2/6-酮基-前列腺素F1α及ALT明显升高，肝细胞形态学发生异常变化；使用L-Arg后，上述指标的异常变化显著减轻，其差异有显著意义（Ｐ＜0.05和Ｐ＜0.01＝。 结论：L－Arg通过提高机体NO水平、降低MDA含量及纠正血栓烷 A2与PGI2的平衡，对HIRI有积极的防治作用。Objective: To explore the protective effect of L-arginine (L-Arg ) on liver during hepatic ischemia-reperfusion injury (HIRI) and its mechanisms. Methods: Changes of several parameters, including nitric oxide (NO), malondiadehyde (MDA), thromboxane B2 (TXB2), 6-keto-prostaglandin F1α(6-keto-PGF1α), TXB2/6-keto-PGF1α(T/K) and alanine aminotransferase (ALT) as well as liver morphological changes, and the effect of L-Arg on them were observed during HIRI in 20 rabbits and 18 patients who were scheduled for elective hepatic surgery. Results: The levels of NO and 6-keto-PGF1α decreased remarkably. MDA concentration, TXB2 content and T/K ratio as well as ALT activity increased significantly. The morphological changes of hepatocytes were obvious during HIRI. After treatment with L-Arg, the variations of all the parameters were markedly alleviated. Conclusion: L-Arg possess preventive effects on HIRI by raising NO and reducing MDA as well as correcting TXA2/PGI2 imbalance after hepatic ischemia-reperfusion.

关 键 词：肝缺血 再灌注损伤 左旋精氨酸 防治 

分 类 号：R575[医药卫生—消化系统]