吡柔比星在骨肉瘤细胞放射增敏中的作用研究  

Study on the effect of pirarubicin in radiosensitization of osteosarcoma cells in vitro

作　　者：许新明[1] 王莉[1] 刘明[1] 徐袁秋[1] 

机构地区：[1]河北医科大学第三医院放疗科,石家庄市050051

出　　处：《河北医药》2014年第2期173-176,共4页Hebei Medical Journal

基　　金：河北省医学科学重点研究课题(编号:20120095)

摘　　要：目的研究吡柔比星在骨肉瘤细胞MG-63的放射增敏中的作用。方法用MTT法检测不同浓度吡柔比星对对数生长期的MG-63细胞的抑制作用,确定IC10的数值,作为实验的药物浓度。用克隆形成分析法测定MG-63细胞在IC10浓度吡柔比星作用24 h后给予不同剂量(0、2、4、6、8Gy)照射以及IC10浓度紫杉醇作用不同时间(12、24、36 h)后给予一定剂量射线照射的存活分数(SF),拟合细胞生存曲线并计算放射增敏比,分析吡柔比星作用于MG-63细胞不同时间(12、24、36 h)后细胞生存率的变化。结果不同浓度吡柔比星作用24 h后,MG-63细胞抑制率逐渐增高(P<0.01),其药物毒性呈浓度依赖性,IC10为0.004μg/ml。IC10浓度吡柔比星増敏比分别1.49(D0比)、1.06(Dq比)和1.04(SF2比),MG-63细胞α/β值为2.23,IC10浓度吡柔比星作用后为0.27。IC10浓度吡柔比星作用于MG-63细胞不同时间后再进行照射,细胞存活分数随时间延长逐渐降低(P<0.01)。结论吡柔比星对MG-63细胞有放射增敏作用,在一定时间内与药物作用时间呈正相关。Objective To investigate the effect of pirarubicin in radiosensitization of human osteosarcoma cells （ MG-63 ） in vitro. Methods M3T was used to measure the inhibitory effect of pirarubicin on MG - 63cells at exponential growth phase to determine IC10 as drug concentration of experiment. After MG-63 were treated with IC10 concentration pirarubicin for 24 hours, the cells were given different doses （0,2,4,6,8Gy） of irradiation as well as IC10 concentration paclitaxel for different time points （12h,24h,36h）, finally the cell survival fraction （SF） was detected by clone formation analysis method,radiosensitization ratio was calculated by fitting cell survival curve, and the changes of cell survival rate of MG-63 treated by pirarubicin in different time points （ 12h,24h, 36 h） were analyzed. Results After 24 - hour pirarubicin administration ,the cell inhibition rate was gradually increased （ P 〈 0.01 ） , and the drug toxicity was dose -dependent, with IC10 concentration being 0. 004p^g/ml. According to DO, Dq and SF2 value, the sensitivity enhancement ratio （SER） of IC10 concentration pirarubicin was 1.49,1.06 and 1.04,respectively, thect/[3 ratio of MG-63 cells was 2.23, after treated with IC10 concentration pirarubicin ,which was 0.27. After treated with IC10 concentration pirarubicin, MG-63 cells received irradiation. The cell survival fraction was gradually decreased with the time prolongation （ P 〈 0.01 ）. Conclusion Pirarubicin has radiosensitizing effect on MG-63 cells,and the effect is positively correlated to effect time of drug within a certain time.

关 键 词：吡柔比星 骨肉瘤 放射增敏 

分 类 号：R730.55[医药卫生—肿瘤]