黄芪甲苷对淀粉样蛋白1-40损伤的胚胎鼠神经干细胞的增殖和分化的影响  

Effects of Astragaloside Ⅳ on the proliferation and differentiation in neural stem cells of Aβ_(1-40) injured embryo mouse

作　　者：胡海燕[1] 金国琴[1] 张学礼[1] 夏花英[1] 许言午[1] 戴薇薇[1] 龚张斌[1] 姚领爱[1] 康湘萍[1] 张丽娜[1] 郑静[1] 

机构地区：[1]上海中医药大学生物化学教研室,上海201203

出　　处：《中华中医药杂志》2014年第1期246-248,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市教委创新项目(No.10YZ53);上海市教育委员会重点学科建设项目(No.J50301)~~

摘　　要：目的:研究黄芪甲苷(AS)对阿尔兹海默病(AD)脑中神经干细胞(NSC)增殖分化的影响。方法:分离胚胎大鼠神经干细胞,并以β淀粉样蛋白1-40(Aβ1-40)处理,MTT法检测AS对细胞增殖的影响;免疫细胞化学检测AS诱导神经干细胞分化的类型;Real-time PCR检测γ-分泌酶及Notch mRNA的表达。结果:高剂量AS能刺激NSC分化,中低剂量AS能够促进细胞增殖,减轻Aβ的细胞毒性作用。机制研究表明,AS既能抑制γ-分泌酶mRNA表达,也能促进Notch mRNA的表达。高剂量AS抑制γ-分泌酶mRNA表达的作用明显,中剂量AS则以促进Notch mRNA的表达为主。结论:高剂量AS能够通过抑制γ-分泌酶mRNA的表达,抑制Notch的激活从而诱导NSC分化。中剂量AS主要通过促进Notch的表达来促进干细胞增殖。Objective： To investigate the effects and molecular mechanism of astragaloside IV （AS） on the proliferation and differentiation in Aβ1-40 injured neural stem cells （NSC）. Methods： Neural stern cells were separated from E13d rat and cultured with Aβ and/or astragaloside IV. The proliferation of NSCs was assayed by MTT. The types of cells were determined by immunocytochemisty. The expression of y-secretase and Notch1 mRNA were measured by Real-time PCR. Results： High dose of AS induced NSC differentiation and inhibited the expression of 7-secretase mRNA. Middle dose of AS promoted NSC growth and increased the level of Notch mRNA. Conclusion： High dose of AS can suppress the Notch signal pathway which determined the NSC differentiation by inhibiting γ-secretase mRNA expression. Middle dose of AS can up-regulate the Notch signal by inducing the Notch mRNA expression.

关 键 词：黄芪甲苷 神经干细胞 Γ-分泌酶 NOTCH 

分 类 号：R285.5[医药卫生—中药学]