血清尿酸测定中维生素C氧化酶抗干扰能力研究  

Research of vitamin C oxidase anti-interference capacity in determination of serum uric acid

作　　者：陈远翔 廖飞[2] 

机构地区：[1]重庆市万州区妇幼保健院,404000 [2]重庆医科大学检验医学院,重庆400016

出　　处：《国际检验医学杂志》2014年第2期208-209,212,共3页International Journal of Laboratory Medicine

摘　　要：目的分析用尿酸酶法测定尿酸时维生素C与维生素C氧化酶之间的量效关系,为临床尿酸试剂盒的选购提供合理化依据。方法用试剂盒测定维生素C氧化酶的活性,配制含不同浓度维生素C氧化酶的尿酸检测试剂。并以维生素C为干扰物,检测不同浓度维生素C氧化酶的抗干扰能力。结果反应体系中维生素C氧化酶浓度为200U/L时,对健康人群(维生素C小于或等于0.04mmol/L)尿酸测定结果没有明显干扰(干扰值-4.2μmol/L<±1.96SD);当维生素C氧化酶浓度升高至400U/L时,对使用维生素C治疗后的人群(维生素C≤0.37mmol/L)尿酸检测值也不会受到显著影响(干扰值-3.0μmol/L<±1.96SD)。结论在选购尿酸试剂时应选用维生素C氧化酶大于或等于400U/L,可以有效防止使用维生素C治疗人群血液中维生素C对检测结果的干扰,使检测值更接近人体血清中真实的尿酸水平。Objective To analyze the concentration response relationship of ascorbic acid and vitamin C oxidase in determination of serum uric acid based on uricase method. In order to provide references for purchase of clinical uric acid reagents. Methods Dif- ferent concentrations of vitamin C oxidase in uric acid reagents were prepared. The ascorbic acid oxidase activity was detected with kit. The anti-interference ability of vitamin C oxidase at various＇ concentration was determined when ascorbic acid as the interference substance. Results In case of vitamin C oxidase concentration was 200 U/L in reaction system,the effect on detection of uric acid （interference value --4.2 μmol/L〈±1. 96SD） for healthy crowd（aseorbic acid C≤0.04 mmol/L） was inconspicuous; and when vitamin C oxidase concentration increased to 400 U/L,the influence on measure of uric acid （interference value 3.0 μmol/L〈 ± 1.96SD） for the ascorhic acid treating group （ascorhic acid C≤0. 37 retool/L） was unohvious either. Conclusion To prevent the interference of blood ascorbic acid for determination of uric acid in the aseorbic acid treating group and make the value of uric acid closed to true level,the uric acid reagents with vitamin C oxidase concentration ≥400 U/L is supposed to be chosen.

关 键 词：抗坏血酸 尿酸 指示剂和试剂 

分 类 号：R446.11[医药卫生—诊断学]