两种不同方法检测肠道71型病毒的比较  

Comparison of two different assays for detection of human enterovirus 71

作　　者：杜昆[1] 李琦[1] 刘学政[1] 

机构地区：[1]湖北省荆州市第一人民医院检验科,434000

出　　处：《国际检验医学杂志》2014年第2期215-216,共2页International Journal of Laboratory Medicine

摘　　要：目的研究不同方法检测肠道71型病毒的灵敏度和特异性。方法采集135例临床确诊为手足口病患儿和44例临床确诊为非手足口病患儿血清,分别用免疫层析法(ICA)和酶联免疫吸附试验(ELISA)检测抗肠道71型病毒IgM抗体。结果ICA法检出阳性病例29例,ELISA法检出阳性病例27例;ICA法和ELISA法的灵敏度分别为21.48%和20.0%,特异性为100%。结论 ICA法和ELISA法均具有较高特异性,但其灵敏度较低。Objective To investigate the sensitivity and specificity of different assays for detection of human enterovirus 71 （EV71）. Methods The sera from 179 patients which were diagnosed as hand foot-and-mouth disease were collected. ICA and ELISA assays were used to detect the serum EV71 IgM antibody. Results 29 patients were identified as EV71 by ICA assay,and 27 patients were identified as EV71 by ELISA assay. The sensitivity and specificity of detection of EV71 with ICA and ELISA were 21.48% and 20.0 %, 100 % and 100%, respectively. Conclusion Both ICA and ELISA assays have a high specificity for detecting EV71 ,while a low sensitivity.

关 键 词：肠道病毒属 敏感性与特异性 酶联免疫吸附测定 

分 类 号：R574[医药卫生—消化系统]