miR-125a-5p对卵巢癌SKOV3细胞增殖和侵袭的影响  

Effects of miR-125a-5p on multiplication and invasion in SKOV3 of ovarian cancer cell

作　　者：刘龙浩[1] 余秋波[2] 魏莎莉[1] 陈渝[1] 刘祖翠[1] 高唐鑫子[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室,重庆400016 [2]重庆医科大学生命科学研究院,重庆400016

出　　处：《解剖学杂志》2013年第6期1009-1012,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（81100443）;重庆市渝中区科技计划项目（20120214）

摘　　要：目的：研究miR-125a-5p对卵巢癌SKOV3细胞增殖和侵袭的影响。方法：通过慢病毒转染技术将含miR-125a-5p的质粒转入SKOV3细胞中，经嘌呤霉素筛选建立稳定表达mi-125a-Sp的SKOV3细胞株。通过实时定量荧光RT-PCR验证miR-125a-5p的表达情况，MTT、流式技术、Transwell、划痕实验检测过表达miR-125a-5p后对SKOV3细胞增殖、凋亡、侵袭及转移能力的影响。结果：过表达miR-125a-5p后，SKOV3细胞增殖能力较对照组有显著提高；流式细胞检测显示过表达miR-125a-5p的SKOV3细胞S期细胞增多，GO／GI期细胞及细胞凋亡率减少；Transwell和划痕实验显示过表达miR-125a-5p的SKOV3细胞侵袭和迁移能力明显高于对照组。结论：SKOV3细胞miR-125a-Sp过表达可能通过减少G0／G1期细胞及细胞凋亡率从而促进细胞增殖、侵袭和迁移能力。Objective： To explore effects of miR-125a-5p on multiplication and invasion in SKOV3 of ovarian cancer ceils. Methods： Using lentivirus transfection technology, plasmids of miR-125a-5p were transformed into SKOV3 and selected by puromycin to establish：SKOV3 cell strains to express miR-125a-Sp steadily. Proving the expressing situation of rniR-125a-Sp by RT-PCR, the proliferation, cell cycle, apoptosis rate, invasion and migration of SKOV3 after the overexpression of miR- 125a-Sp were analysed by MTq＇, flow cytometric （FCM）, transwell and scratch test. Results： The ability of proliferation of SKOV3 was improved obviously compared with the control group after overexpression of miR-125a-Sp; the S-phase of the cells after the overexpression of miR-125a-Sp was increased obviously, G0/G1 phase and the apoptosis rate were decreased; transwell and scratch test results indicated that the cells after the overexpression of miR-125a-Sp had a better invasion and migration ability than did the control group. Conclusion： SKOV3 after overexpression of miR-125a-Sp may promote itself to improve the ability of proliferation, invasion and migration by decreasing G0/G1-phase calls and the apoptosis rate of cells.

关 键 词：miR-125a-5p 过表达 卵巢癌SKOV3 增殖 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]