兰州回族人群STR基因座多态性及遗传关系  

Genetic polymorphisms of STR loci and genetic relationship in Hui ethnic group from Lanzhou

作　　者：孙红兵 杨鑫 哈飞 张子龙 谢小冬[1,3] 

机构地区：[1]甘肃省证据科学技术研究与应用重点实验室,兰州730070 [2]兰州市公安局刑事科学技术研究所,兰州730030 [3]兰州大学基础医学院遗传学研究所,兰州730000

出　　处：《解剖学杂志》2013年第6期1098-1101,1125,共5页Chinese Journal of Anatomy

基　　金：甘肃省青年科技基金计划（1208IL1YA073）;兰州市科技计划基金（2012-1-22）.

摘　　要：目的：通过研究兰州地区500名回族无关个体D8S1179等15个基因座的遗传频率，同时应用其他9组中国人群的STR基因座数据进行分析，了解各人群的群体遗传结构及遗传关系。方法：对500名兰州回族个体血样DNA进行15个SIR基因座的复合扩增并检测；分析兰州回族等10组人群的遗传亲源系数并构建群体系统发生树。结果：兰州回族15个STR基因座均符合Hardy-Weinberg平衡定律，联合应用此15个S1R基因座的累计个体识别能力（TDP）达0．999999999999999986，累计非父排除率（TPE）达0．999995813，符合个人识别及亲权鉴定的统计学要求；遗传关系分析显示兰州回族与广西回族的遗传关系最接近，并与青海回族、新疆维吾尔族、甘肃裕固族、青海撒拉族列为1个聚类群，兰州回族与兰州汉族较其他8个民族遗传关系最远。结论：兰州回族STR基因座遗传多态性数据为涉及回族人群的司法鉴定工作提供了基础数据，同时这些数据的获得将有助于进行民族、起源、迁徙、基因交流等群体遗传关系的研究。Objective： To study the STR polymorphisms information of 500 Hui unrelated individuals from Lanzhou and calchlate the ge/ietic similarity coefficient and genetic distance of Lanzhou Hui population and other Chinese ethnic groups in different areas. Methods ：The genotypes of 15 STR loci in Lanzhou Hui population were generated by multiplexing PCR and genotyping. The genetic similarity coefficient and genetic distance were calculated. Results： All loci met Hardy-Weinberg ;,equilibrium expectations, i：he statistical／ahalysis showed the cumulated power of discrimination （TDP） was 0. 999 999 999 999 999 986, the cumulated power of exclusion （TPE） was 0. 999 995 813, which i matched the order of requirements for pergonal identification and paternity testing. Meanwhile, the genetic distances showed that Lanzhou Hui population and Guangxi Hui population were in the same clade, and the genetic distances among Lanzhou Hui, Guangxi Hui, Qinghai Hui, Xiniiang Uyghui, nsu YugUr and Qinghai Sala were the shortest in all of ten populations in different areas in China. The distance between Lanzhou Hui population and Lanzhou Han population was the fastest.

关 键 词：兰州回族 STR基因座 多态性 遗传关系 

分 类 号：R394[医药卫生—医学遗传学]