锂盐促进大鼠前腭缝扩张成骨中血管形成的研究  

Lithium Enhances Angiogenesis and Osteogenesis in the Expanding Premaxillary Suture in Rats

作　　者：郭佳佳[1] 张薇[1] 江燕[1] 唐国华[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔正畸科.上海市口腔医学重点实验室,上海200011

出　　处：《口腔医学研究》2014年第1期1-4,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81271113)

摘　　要：目的:观察锂盐对大鼠前腭缝扩张后血管形成的影响。方法:扩大簧扩张SD大鼠前腭缝,实验组于扩张前7d开始每天注射氯化锂,对照组注射氯化钠,扩张后2、5、7d处死。免疫组化检测β-catenin表达,CD31、vWF抗体检测血管形成,钙黄绿素标记新骨形成。结果:β-catenin在成骨细胞和血管内皮细胞中表达,实验组β-catenin信号增强,同时血管形成显著增多,7d后新骨量明显增加。提示通过增强β-catenin信号促进血管形成,是锂盐促进大鼠前腭缝牵张成骨的途径之一。Objective： To identify the effect of lithium on angiogenesis in the expanding premaxillary suture in rats. Methods： SD rats were subjected to premaxillary suture expansion with a helix spring. The experimental animals were given a daily intraperitoneal injection of lithium chloride 7 days before the expansion. The control rats were in- jected with sodium chloride. All the animals were sacrificed on 2d, 5d and 7d after the expansion. [^--eatenin ex- pressions were evaluated by immunohistoehemieal staining. CD31 and vWF antibodies were used to detect the anglo- genesis. Calcein was used to label new bone formation. Results： ]3--catenin expressions were detected in both osteo- blasts and endothelial cells in the expanding premaxillary suture. The administration of lithium chloride led to an el- evation of ^--catenin expression and an increase in the number of vessels. Meanwhile, there was a significantly in- crease in the amount of new bone formation after lithium treatment on day 7. Conclusion： Lithium stimulates bone formation in the expanding premaxillary suture may through, at least in part, promoting angiogenesis by activation of β-catenin signaling.

关 键 词：氯化锂 Β-CATENIN 血管形成 前腭缝 牵张成骨 

分 类 号：R363[医药卫生—病理学]