JAZF1基因对3T3-L1脂肪细胞Akt和AMPK信号途径的影响  

Effect of JAZF1 on Akt and AMPK signal pathway in 3T3-L1 adipocytes

作　　者：罗小河[1] 李伶[1] 杨刚毅[2] 杜林[1] 贾彦军[1] 冉文侠[1] 张亚丽[1] 罗妤[1] 

机构地区：[1]重庆医科大学检验系临床生化教研室和教育部临床检验诊断学重点实验室,400016 [2]重庆医科大学附属第二医院内分泌科

出　　处：《中华内分泌代谢杂志》2014年第1期61-64,共4页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金项目（30771037,81070640,30871199,30971388）;教育部博士点基金（20105503110002）;重庆市自然科学基金重点项目（cstc2012jjB10022）

摘　　要：目的探讨JAZFl（juxtaposed with another zinc finger gene 1）基因对333一L1脂肪细胞蛋白激酶B（Akt）和AMP活化的蛋白激酶（AMPK）信号通路的影响。方法构建pGenesil—JAZFlshRNA重组质粒，转染3T3．L1脂肪细胞后分别用胰岛素或利拉鲁肽处理细胞。2．脱氧-^3H—D-葡萄糖掺入法检测细胞的葡萄糖摄取，Western印迹法检测各组细胞Akt和AMPK的磷酸化水平。结果成功构建的pGenesil-JAZFlshRNA重组载体转染333-L1脂肪细胞后下调JAZF1表达，使基础和胰岛素刺激的葡萄糖摄取降低33．6％和38．8％（均P〈0．05）。胰岛素或利拉鲁肽增加333-L1脂肪细胞Akt和AMPK磷酸化水平（均P〈0．05），JAZF1表达下调降低基础和2种激素刺激的Akt和AMPK活性（均P〈0．05）。结论JAZF1基因可能通过Akt和AMPK信号通路改善糖代谢。Objective To explore the effect of juxtaposed with another zinc finger gene 1 （ JAZF1 ） on protein kinase B （Akt） and AMP-activated protein kinase （AMPK） signal pathway in 3T3-L1 adipocytes. Methods Recombinant pGenesil-JAZF1 shRNA plasmids were constructed. 3T3-L1 adipoeytes transfected with pGenesil-HK or pGenesil-JAZF1 plasmid were treated with insulin or liraglutide. Glucose uptake was detected by 2-deoxy-3H- glucose. The pbosphorylations of Akt and AMPK in 3T3-L1 adipocytes were detected by Western blot. Results Recombinant pGenesil-JAZFlshRNA plasmids was transfected into 3T3-L1 adipocytes and JAZFI expression was downregulated, resulting in decreased basal and insulin-stimulated glucose uptake by 33.6% and 38.8% （ both P〈 0.05 ）. Insulin and liraglutide markedly increased the phosphorylation levels of Akt and AMPK in 3T3-L1 adipocytes （P 〈 0. 05 ）. The downregulation of JAZF1 expression decreased basal and this two hormones-stimaulted phophorylations of Akt and AMPK （ all P〈0.05）. Conclusion JAZF1 gene may improve glucose metabolism through Akt and AMPK signal pathway.

关 键 词：JAZF1 蛋白激酶B AMP活化的蛋白激酶 胰岛素 利拉鲁肽 

分 类 号：R363[医药卫生—病理学]