再灌注前干预肥大细胞功能对大鼠肠缺血再灌注后早期肝损伤的影响  

Effect of intervention for mast cell function before reperfusion on intestinal ischemia-reperfusion-induced early liver injury in rats

作　　者：黄品婕[1] 李晓芸[1] 罗晨芳[1] 张瑷兰[1] 刘健培 

机构地区：[1]中山大学附属第三医院麻醉科 [2]胃肠外科,广东广州510630

出　　处：《中国病理生理杂志》2014年第1期85-90,共6页Chinese Journal of Pathophysiology

基　　金：广东省医学科学基金资助项目(No.B2012123);广东省自然科学基金博士启动项目(No.S2013040016160)

摘　　要：目的:探讨肠缺血后再灌注前干预肥大细胞功能对SD大鼠继发肝脏损伤的影响及机制。方法:35只大鼠随机分成5组:假手术组(S组)、缺血再灌注组(IR组)、缺血再灌注+色甘酸钠组(IR+C组)、缺血再灌注+酮替芬组(IR+K组)和缺血再灌注+compound 48/80组(IR+CP组)。复制大鼠肠缺血再灌注模型,IR+C、IR+K和IR+CP组分别在再灌注前5 min经尾静脉给予相应药物,S和IR组给予等量生理盐水。再灌注4 h后处死大鼠,观察各组肝脏病理变化及评分,测定血清丙氨酸氨基转移酶(ALT)活性、天冬氨酸氨基转移酶(AST)活性和组胺含量,检测肝脏乳酸脱氢酶(LDH)活性、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和肿瘤坏死因子α(TNF-α)、白细胞介素8(IL-8)水平。结果:与S组相比,IR组肝脏病理损伤明显(P<0.05),血清ALT、AST水平、组胺含量、肝LDH活性、MDA、TNF-α、IL-8水平升高,SOD活性减弱(P<0.05)。与IR组相比,IR+C和IR+K组肝脏损伤减轻,以上指标呈相反趋势(P<0.05),IR+CP组则加重肝损伤及恶化检测结果(P<0.05)。结论:再灌注前抑制肥大细胞功能可以减轻肠缺血再灌注后早期肝脏损伤,其机制可能与组胺释放、氧化损伤和炎症反应有关。AIM. To explore the effect of intervention for mast cell function before reperfusion on intestinal is- chemia-reperfusion （IR）-induced early liver injury. METHODS： Adult SD rats （n = 35 ） were randomized into 5 groups with 7 rats each t sham operation group （ S group）, IR group, cromolyn sodium treatment group （ IR ＋ C group, 25 mg/ kg）, ketotifen treatment group （IR ＋ K group, 1 mg/kg）, compound 48/80 treatment group （IR ＋ CP group, 0.75 mg/ kg）, IR was induced by superior mesenteric artery occlusion for 75 min followed by 4 h of reperfusion. The agents were in- travenously administered 5 rain before reperfusion. The serum levels of aspartate aminotransferase （ AST）, alanine amin- otransferase （ALT） and histamine, and the liver levels of lactate dehydrogenase （LDH）, tumor necrosis factor ot （TNF- or）, interleukin-8 （IL-8）, malondialdehyde （MDA） and superoxide dismutase （SOD） were assessed. The liver his- topathologic changes were also evaluated. RESULTS： IR resulted in severe liver injury as demonstrated by great increases in injury scores, concomitant significant increases in serum levels of AST, ALT and histamine, and liver levels of LDH, TNF-ct, IL-8, and MDA, accompanied by reduced SOD activity （all P 〈 0.05 vs S group）. Treatment with cromolyn sodi- um or ketotifen markedly alleviated IR-mediated liver injury as confirmed by significant reduction of the above biomedical changes, whereas compound 48/80 further aggravated liver injury by dramatically enhancing the biomedical changes （ all P 〈0.05 vs IR group）. CONCLUSION： Inhibition of mast cell function before reperfusion may reduce early liver injury in- duced by intestinal ischemia reperfusion. Histamine, oxidative stress and inflammatory response may provide promising effects on it.

关 键 词：肥大细胞 肠缺血再灌注 肝损伤 

分 类 号：R363[医药卫生—病理学]