姜黄素类化合物提高线虫的抗氧化应激能力  

Effect of curcuminoids on oxidative stress resistance in Caenorhabditis elegans

作　　者：何露[1] 李中[1] 韦睿[1] 雷清锋[1] 赵云燕[1] 

机构地区：[1]中山大学附属第六医院神经科,广东广州510655

出　　处：《中国病理生理杂志》2014年第1期154-158,共5页Chinese Journal of Pathophysiology

基　　金：广东省医学科学研究基金资助项目(No.A2012211)

摘　　要：目的:研究姜黄素类化合物姜黄素(Cur)、去甲氧基姜黄素(DMC)和双去氧基姜黄素(BDMC)在秀丽隐杆线虫体内的抗氧化应激作用并探讨其作用机制。方法:应用胡桃醌诱导,观察姜黄素类化合物对线虫在氧化应激状态下生存率的影响;用分子探针2',7'-二氯二氢荧光素二乙酸酯(H2DCF-DA)观察姜黄素类化合物对线虫氧化应激状态下体内活性氧(ROS)的影响;应用荧光显微镜及图像软件分析姜黄素类化合物对线虫氧化应激相关蛋白谷胱甘肽S-转移酶4(GST-4)表达的影响。结果:经姜黄素类化合物预处理后,氧化应激条件下线虫的生存率明显提高;姜黄素类化合物显著抑制了氧化应激状态下线虫体内的ROS水平;在氧化应激条件下,Cur处理组及DMC处理组线虫的GST-4表达量均显著增高(P<0.05)。结论:姜黄素类化合物可能通过降低线虫体内的ROS水平、上调特定应激蛋白GST-4的表达而增强其抗氧化应激能力。AIM： To examine the effect of curcuminoids, including curcumin （ Cur）, demethoxycurcumin （DMC） and bisdemethoxycurcumin （BDMC）, on oxidative stress resistance in Caenorhabditis（C.） elegans. METH- ODS： C. elegans strain N2 was used in the study and divided into control group and curcuminoids treatment groups. The survival of the worms under juglone-induced oxidative stress was measured. Intracellular reactive oxygen species （ROS） was determined in treated and control C. elegans strain N2 using 2＇,7＇-dichlorofluorescein diacetate （H2DCF-DA）. To study the effect of cureuminoids on the expression of glutathinne S-transferase 4 （ GST-4）, the C. elegans strain CL2166 （ GST-4： ：GFP： ：NLS） was used. The fluorescence images of GFP were captured by fluorescence microscope in treated and control C. elegans and quantified by Image-Pro Plus software. RF_~ULTS： Pretreatment with Cur, DMC or BDMC signifi- cantly increased the survival of the worms exposed to juglone-induced oxidative stress. Accumulation of ROS in the worms after oxidative stress was prevented by the treatment with curcuminoids. Moreover, the expression of antioxidative-related protein GST-4 was significantly induced by Cur and I）MC. CONCLUSION： Cureuminoids exert significant in vivo antioxi- dant activity against juglone-induced oxidative stress in C. elegans, which may associate with the attenuating ROS accumu- lation under the condition of oxidative stress and the increase in the expression of antioxidant-related protein GST-4 in the

关 键 词：姜黄素类化合物 氧化性应激 秀丽隐杆线虫 谷胱甘肽 s-转移酶4 

分 类 号：R285[医药卫生—中药学]