Sombati细胞模型中Toll样受体4的表达及意义  

Expression and significance of TLR4 in Sombati's cell-based model of epilepsy

作　　者：李偲俊[1] 吴原[1] 黄金山[1] 叶洁梅[1] 韦兴[1] 刘夕霞[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁530021

出　　处：《中国病理生理杂志》2014年第1期175-179,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81160167)

摘　　要：目的:研究Sombati细胞模型中神经元Toll样受体4(TLR4)表达变化,探讨TLR4在Sombati癫痫细胞发病过程中的意义。方法:将新生SD乳鼠海马神经元进行体外培养至第9天,随机分为对照组和Sombati细胞模型组,分别检测TLR4蛋白及TLR4 mRNA的表达。结果:免疫组化结果显示Sombati细胞TLR4蛋白表达增强,荧光定量PCR显示Sombati细胞组TLR4 mRNA表达增加(P<0.05),且随时间的延长而增强。结论:Sombati细胞模型中TLR4 mRNA与蛋白表达均增加,可能与癫痫的发病有关。AIM ： To study the change of Toll-like reporter 4 （TLR4） expression in Sombati＇s cell-based model of epilepsy and to explore the role of TLR4 in this model. METHODS ： After cultured in vitro for 9 d, the neurons of new- born SD rats were randomly divided into control group and model group. The neurons in model group were cultured in low- magnesium medium for 3 h and then returned to the normal medium culture. The expression of TLR4 at mRNA and protein levels was detected by fluorescence quantitative PCR and the method of immunohistochemistry, respectively. RESULTS： The results showed that the protein expression of TLR4 was enhanced in the Sombati＇ s cells. The re- suits of fluorescence quantitative PCR showed that the mRNA expression of TLR4 time-dependently increased in the Sombati＇s cells （P 〈 0.05 ）. CONCLUSION： The expression of TLR4 in the neurons is up-regulated in Sombati＇s cell- based model of epilepsy in rats, suggesting that TLR4 is associated with the pathogenesis of epilepsy.

关 键 词：癫痫 神经元 TOLL样受体4 

分 类 号：R742.1[医药卫生—神经病学与精神病学]