新疆汉族、维吾尔族HPV16E6基因变异与子宫颈癌及宫颈病变的关系  

The relationship of HPV16E6 mutation to cervical lesions and cervical carcinoma in Han and Uygur in Xinjiang

作　　者：古丽娜尔.阿不拉 朱开春[1] 玛依努尔.尼牙孜 马正海[2] 

机构地区：[1]新疆维吾尔自治区人民医院妇科,新疆乌鲁木齐830001 [2]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《基础医学与临床》2014年第1期11-15,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81160317);自治区人民医院院内科研基金(20080110)

摘　　要：目的探讨新疆地区汉族、维吾尔族宫颈癌及宫颈病变中HPV16型E6基因变异分布及频率,比较两个民族之间的差异。方法对140例HPV16阳性标本,设计E6基因特异性引物,通过PCR扩增HPV16E6全长基因,PCR产物直接测序,进行序列分析并与德国标准株对比,筛选突变位点。结果 123例汉族、维吾尔族妇女宫颈癌及宫颈病变标本中均存在HPV16E6变异株,其中突变率分别为:47.37%(27/57)和50%(33/66),E6突变位点主要是L83V、D25E、D64E、I73V、H78Y、D113E,其中L83V变异株在两个民族突变中占的比例均最高,汉族L83V突变频率为29.82%(17/57)显著低于维吾尔族的40.90%(27/66)(P<0.05)。而汉族D25E突变频率为19.30%(11/57)显著高于维吾尔族的7.58%(5/66)(P<0.05),D64E变异株在维族突变株中占的比例为6.1%,而汉族标本中未检出。结论两个民族HPV16 E6基因变异发生的位置和变异发生的频率存在差异,维吾尔族人群中HPV16E6基因D64E变异株可能与该地区维吾尔族宫颈癌高发存在一定的关系。Objective To investigate distribution of HPV16E6 mutation and whether there are differences in Han, Uygur cervical cancer（ CC） and precancerous lesions. the relationship between the mutation and high incidence of CC in Han and Uygur women. Methods 140 precancerous lesions samples and cervical carcinoma patients with HPV16 genotypes was chosen and HPV16E6 gene was amplified by polymerase chain reaction（PCR）. The PCR fragments were sequenced and analyzed. Results There was mutations in 123 cases,the distribution in Han and Uygur were 47. 37%（ 27 /57） and 50%（ 33 /66） respectively. They were positive for mutations D25E, D64E, I73V, H78Y,D113E,L83V,the hot mutation was L83V with rates was 29. 82%（17 /57）in Han and 40. 90%（27 /66） in Uygur,Rates was D25E 19. 30%（11 /57）in Han and 7. 58%（5 /66）in Uygur. Rates of D64E 6. 1% was only found in Uygur. Conclusions There was difference between HPV16E6 gene mutation sites and rates in two na-tions. Our research suggested that the distribution of D64E in Uygur might be associated with higher incidence of CC in this population.

关 键 词：汉族 维吾尔族 宫颈癌及宫颈病变 人乳头瘤病毒 E6基因 

分 类 号：R737.33[医药卫生—肿瘤]