苯丙酸诺龙促进胰岛NIT-1细胞Nampt表达和胰岛素分泌  

Benzyl propionate nandrolone enhances Nampt expression and insulin secretion in NIT-1 islet cells

作　　者：陈洁润 吴雅婷[1] 冯乔[1] 沈旺[1] 冯乐平[1] 

机构地区：[1]桂林医学院生物技术学院,广西桂林541004

出　　处：《基础医学与临床》2014年第1期68-71,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30860116;31060161);广西科技厅科研基金(桂科自0728230);广西教育厅2013年自治区大学生创新创业训练计划立项项目〔桂教高教(2013)14号〕;广西教育厅科研资助课题〔桂教科研(2010)10号;201010LX325;201010LX326〕

摘　　要：目的探讨雄性激素苯丙酸诺龙在氧化应激条件下对胰岛细胞的作用,为2型糖尿病治疗提供理论依据。方法将培养的NIT-1细胞按不同葡萄糖浓度(5.6、11.1、16.7和27.6 mmol/L)分为4组,分别用10μg/mL苯丙酸诺龙处理48 h,之后用流式细胞仪检测细胞周期;用Western blot检测Nampt和FOXO1蛋白表达和用放射免疫法测定细胞胰岛素分泌。结果用苯丙酸诺龙处理48 h后,1)低糖条件下细胞G0/G1期阻滞明显改善(P<0.01);2)高糖条件下细胞G2/M期阻滞得到缓解(P<0.01);3)高糖氧化应激状态下,苯丙酸诺龙促进胰岛细胞Nampt表达(P<0.05)和抑制非磷酸化的FOXO1蛋白表达(P<0.01),细胞分泌胰岛素增加。结论苯丙酸诺龙能够在高浓度葡萄糖条件下改善胰岛细胞生存状况和促进胰岛素分泌,这些效应可能与促进细胞内Nampt表达和抑制FOXO1密切相关。Objective To discuss the effects of nandrolone styrene acrylic acid on islet cells under the condition of oxidative stress for the theoretical basis of the treatment of type 2 diabetes. Methods NIT-1 cell were divided into four groups with different concentrations of glucose（5. 6,11. 1,16. 7 and 27. 6 mmol / L）,then treat with 10μg/mL Benzyl propionate nandrolone for 48 h. Nampt and FOXO1 protein expression was detected by Western blot assay. Cell cycle was determined by FCM and cell insulin secreted level was measured with radioimmuno-assay. Results Treated with benzyl propionate nandrolone for 48 h,1）G 0 / G 1 phase retardation effect of the cell cycle was relieved（P 〈0. 01）when the cell cultured in lower energy,but 2）G 2 / M block effect of the cell significantly remitted（P 〈0. 01）under the condition of high glucose concentration. 3） Benzyl propionate nandrolone can promote Nampt protein expression（ P〈 0. 05） and promoted insulin secretion,inhibiting the dephosphorylated FOXO1 expression（P 〈0. 01）. Conclusion Benzyl propionate nandrolone would promote islet NIT-1 cell proliferation and insulin se-cretion. These effects are closely related to the inhibition of FOXO1 inhibit of FOXO1 through Nampt increase.

关 键 词：胰岛NIT-1细胞 苯丙酸诺龙 NAMPT FOXO1 细胞周期 

分 类 号：R587.1[医药卫生—内分泌]