食欲素A促人胃癌SGC7901细胞凋亡  

Orexin A promotes the apoptosis of human gastric cancer cell SGC7901

作　　者：高之峰[1] 张建福[2] 费素娟[1] 汪诗卉[1] 董秋菊[1] 韩红霞[1] 

机构地区：[1]徐州医学院附属医院消化内科,江苏徐州221002 [2]徐州医学院生理学教研室,江苏徐州221002

出　　处：《基础医学与临床》2014年第1期98-103,共6页Basic and Clinical Medicine

摘　　要：目的观察食欲素A对胃癌SGC7901细胞生长的影响,并初步探讨其机制。方法常规培养胃癌SGC7901细胞,单独食欲素A处理及食欲素A受体拮抗剂SB408124干预后再用食欲素A作用,MTT法检测药物对SGC7901细胞抑制率;Hoechst33258荧光染色法及流式细胞术检测细胞凋亡;Western blot方法检测BCL-2、BAX、Cyt c和活化caspase-3蛋白的表达。结果食欲素A呈剂量-时间依赖性的抑制胃癌SGC7901细胞增殖,其中1.0μmol/L食欲素A作用24h对胃癌细胞增殖的抑制作用最强,抑制率为65.32%±2.51%(P<0.01);食欲素A组可见较多凋亡细胞:胞核或胞质内可见浓染致密的蓝色荧光,有明显的核固缩,SB408124干预后,凋亡细胞数量明显减少(P<0.05);食欲素A作用后,凋亡率为59.52%±1.38%,而SB408124干预后,凋亡率降至38.96%±1.82%(P<0.05);食欲素A可上调凋亡相关蛋白BAX、Cyt c及活化caspase-3的表达,下调抗凋亡蛋白BCL-2的表达(P<0.05),SB408124干预后,食欲素A作用减弱。结论食欲素A对胃癌SGC7901细胞生长具有明显的抑制增殖和促凋亡作用;食欲素A的作用是通过其选择性受体实现的。Objective To explore the effect of orexin A on the growth of human gastric cancer cell line SGC7901 and its mechanisms. Methods The SGC7901 cells were treated by orexin A and SB408124（an antagonist of orexin A re-ceptor subtype 1）. The MTT assay was used to detect growth inhibitory rates of orexin A on human gastric cell SGC7901. The apoptosis was determined by Hoechst 33258 fluorochrome staining and flow cytometric analysis; and we used Western blot to detect the expressions of BCL-2 BAX Cyt c and caspase-3. Results Orexin A can inhibit the growth of SGC7901 cells. This can be proved by a direct relationship between a longer time for cells to culture when given a higher dose of orexin A. The strongest inhibitory rate was 65. 32% ± 2. 51%,when we used 1. 0μmol/L orexin A treat for 24 h（P 〈0. 01）; we can see many apoptotic cells that the nuclear condensation in orexin A group, While cells that were pretreated with SB408124 showed the number of the native cell was decreased（P 〈0. 05）. After treated with orexin A,the apoptosis rate was 59. 52% ± 1. 38%,but when we pretreated with SB408124,the apoptosis rate decrease to 38. 96% ± 1. 82%;the expressions of BAX Cyt c and caspase-3 protein were increased,but the expression of BCL-2 was decreased（P 〈0. 05）. As mentioned above,the inhibitory effects of orexin A on SGC7901 cell were weakened by the pretreatment with SB408124. Conclusions Orexin A significantly inhibits the growth of SGC7901 cells through inducing cell apoptosis. Its molecular mechanism is associated with OX1R.

关 键 词：食欲素A SGC7901细胞 SB408124 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]