HIF-1α/VEGF在肝细胞癌的表达及其与肿瘤血管生成的关系  

Expression of HIF-1α and VEGF in hepatocellular carcinoma and correlation with tumor angiogenesis

作　　者：熊义富[1] 李丽[2] 徐浩[1] 刘康[1] 张小明[1] 王琼[2] 李祖茂[2] 杨林[1] 

机构地区：[1]医学影像四川省重点实验室.川北医学院附属医院放射科 [2]川北医学院附属医院病理科,四川南充637000

出　　处：《川北医学院学报》2013年第6期530-533,共4页Journal of North Sichuan Medical College

基　　金：四川省科技厅项目(2008JY0088-2);四川省教育厅重点项目(07ZA030)

摘　　要：目的:探讨缺氧诱导因子-1α(hypoxia-inducible factor,HIF-1α)和血管内皮生长因子(vascular endothelial growth factor,VEGF)在肝细胞癌(hepatocellular carcinoma,HCC)的表达及其与肿瘤血管生成的关系。方法:采用免疫组织化学SP法检测41例HCC组织及16例非肿瘤肝组织的HIF-1α及VEGF表达,采用鼠抗人CD31单克隆抗体标记肿瘤血管内皮细胞,计数HCC组织微血管密度(micro-vessel density,MVD)值。结果:在HCC组织与非肿瘤肝组织中,HIF-1α、VEGF阳性表达率和MVD值分别为82.9%、80.5%、41.0±20.2与37.5%、25.0%、20.1±9.1,两组差异均具有统计学意义(P<0.05)。在HCC组织中,HIF-1α、VEGF与MVD三者之间均具有相关性,并与病理分级相关(P<0.05)。结论:在HCC组织中,HIF-1α和VEGF呈高表达,与肿瘤血管生成密切相关。Objective：Explore and discuss the expression of hypoxia-inducible factor-1 α（ HIF-1 α） and vascular endothelial growth factor （VEGF） in hepatocellular carcinoma as well as their correlations with tumor angiogenesis. Methods ：The HIF-1α and VEGF ex- pression in Forty-one cases of HCC tissue samples and 16 cases of nontumorous hepatic tissue samples were analyzed by immunohisto-chemistry streptavidin-peroxidase （SP） method. The anti-human mouse CD31 monoclonal antibody was adopted to mark the tumor vas- cular endothelial cells and assess the micro-vessel density （MVD）. Results：The positive expression rates of HIF-1α and VEGF were 82.9% （34/41）,37.5% （6/16）,80.5% （33/41） and 25.0% （4/16） and the MVD values were 41.0 ±20.2 and 20.1 ±9.1 in HCC tissues and adjacent noncancerous tissues respectively;the differences between the two groups were statistically significant for all three variables （P 〈 0. 05 ）. The expression levels of HIF-1α ,VEGF and the MVD value are correlated with each other in HCC tissues. Moreover,HIF-1α and VEGF expression,as well as the MVD value,were correlated with the pathological degree of HCC. Conclusion： HIF-1 α and VEGF are frequently over expressed in HCC,and play an important role in tumor angiogenesis.

关 键 词：肝细胞癌 缺氧诱导因子-1Α 血管内皮生长因子 微血管密度 血管生成 

分 类 号：R734.2[医药卫生—肿瘤]