不同直径球囊对建立未成年兔腹主动脉狭窄模型的影响  

Impact of balloon size on establishment of abdominal aortic stenosis models in young rabbits

作　　者：耿化晓 计晓娟[1] 余更生[1] 郭军[2] 翟秀全[1] 李军帅[1] 

机构地区：[1]重庆医科大学附属儿童医院心脏中心儿童发育疾病研究省部共建教育部重点实验室儿科学重庆市重点实验室重庆市儿童发育重大疾病诊治与预防国际科技合作基地,重庆400014 [2]绵阳市中心医院儿科,四川绵阳621000

出　　处：《中国介入影像与治疗学》2014年第2期112-115,共4页Chinese Journal of Interventional Imaging and Therapy

基　　金：重庆市自然科学基金(CSTC2009BB5267);重庆市卫生局课题(2011-2-242);重庆市中医药卫生局课题(2011-2-57)

摘　　要：目的探讨不同直径球囊对建立未成年兔腹主动脉狭窄模型的影响。方法雄性新西兰大白兔25只,随机分为正常对照组(n=5)和建模组(n=20),建模组随机平均分为建模A、B、C、D组,分别采用直径2.5、3.0、3.5、4.0mm的球囊扩张损伤兔腹主动脉。术后28天高频超声评价血管直径狭窄率,同时观察内膜增生情况。结果建模A、B、C组建模后28天超声测量直径狭窄率高于正常对照组和建模D组(P<0.05)。各建模组建模后28天新生内膜面积和内膜增生指数较正常对照组增加(P<0.01),建模A、B、C组球囊血管直径比例与内膜增生指数呈正相关(r2=0.784,P<0.001)。建模D组球囊血管直径比例最高,但新生内膜面积和内膜增生指数低于建模C组(P<0.05)。结论选用适当大小球囊,通过内膜损伤建立未成年兔腹主动脉狭窄模型是安全可行的。Objective To estimate the impact of balloon size on establishment of young rabbit models of abdominal aortic stenosis. Methods Twenty-five New Zealand white rabbits were randomly divided into normal control group （n=5） and operated groups （n=20）. Rabbits in operated groups were averagely divided into group A, B, C and D, matching to balloon diameters of 2.5, 3.0, 3.5 and 4.0 mm. Stenosis degree was explored with high-frequency ultrasound, and intimal hyperplasia was examined with pathology 28 days after balloon-injury. Results Stenosis ratio of group A, B and C were higher than that of normal control group and group D 28 days after balloon-injury （P（0.05）. Neointimal area and intimalmedia ratio increased in the operated groups compared with normal control group （P〈（0.01）. Positive correlation was not- ed between the degree of vessel expansion and proliferation index （r2 =0. 784, P〈0. 001） among group A, B and C. The degree of vessel expansion in group I） was the highest, but neointimal area and intimal-media ratio of group D were smaller than those of group C （P〈0.05）. Conclusion It is feasible to found young-rabbit models of abdominal aortic stenosis with appropriate balloon by intimal-injury technique.

关 键 词：兔 主动脉 腹的 模型 动物 

分 类 号：R-332[医药卫生] R543.1