伊曲康唑对Lewis肺癌荷瘤裸鼠恶性胸腔积液生成的抑制作用  

Itraconazole suppresses malignant pleural effusion in nude mice with lewis lung carcinoma

作　　者：王允芬[1] 姚艳雯[1] 刘红兵[1] 吕镗烽[1] 袁冬梅[1] 宋勇[1] 

机构地区：[1]南方医科大学临床学院(南京军区南京总医院)呼吸与危重症医学科

出　　处：《医学研究生学报》2014年第1期19-22,共4页Journal of Medical Postgraduates

基　　金：江苏省自然科学基金(BK2011658)

摘　　要：目的恶性胸腔积液(malignant pleural effusion,MPE)是晚期肺癌患者常见并发症,预后极差。研究拟通过建立MPE裸鼠模型来观察伊曲康唑对MPE生成的抑制作用。方法采用胸腔种植绿色荧光标记Lewis肺癌(green fluorescent protein-Lewis lung carcinoma,GFP-LLC)细胞的方法建立荷瘤裸鼠模型。3组实验小鼠分别给予胸腔注射25 mg/kg伊曲康唑溶液(H组)、8 mg/kg伊曲康唑溶液(L组),并以注射羟丙基β-环糊精溶液作为对照(C组)的处理方法。每3天处理1次,共4次。使用CT扫描方法观察小鼠胸腔积液形成情况。实验结束处死全部裸鼠,测量胸腔积液体积及血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)浓度。结果伊曲康唑对荷瘤裸鼠胸腔积液体积及胸腔积液VEGF-C浓度均有抑制作用(P<0.05),且抑制呈浓度依赖性。结论伊曲康唑能抑制荷瘤裸鼠胸腔积液中VEGF-C表达,并可能成为一种有效的抑制肿瘤淋巴管生成药物。Objective Malignant pleural effusion （MPE） is a common complication in advanced lung cancer patients with a poor prognosis. The aim of this study was to observe the inhibitory effect of itraconazole on MPE by establishing a model of MPE in nude mice. Methods The model of MPE was established in nude mice by injecting lewis lung carcinoma cells expressing green fluo- rescent protein （LLC-GFP） into the pleural cavity. Then the mice were divided into three groups and treated with high-dose （25 mg/kg） itraconazole, low-dose itraconazole（8 mg/kg）, and hydroxypropyl-13-cyclodextrin （H-13-C）, respectively, 4 times with a 3- day interval. The formation of MPE was observed by transverse CT imaging. All the mice were sacrificed finally for the measurement of the MPE volume and the concentration of vascular endothelial growth factor C （VEGF-C） in MPE by ELISA. Results Itraconazole significantly inhibited both the MPE volume and the expression of VEGF-C in MPE in a dose-dependent manner （ P 〈 0.05 ）. Conclusion Itraconazole, with its inhibitory effect on the expression of VEGF-C in the nude mouse model of MPE, may become an ef-fective dru for suooressin tumor lvmohanioenesis.

关 键 词：伊曲康唑 非小细胞肺癌 恶性胸腔积液 血管内皮生长因子C 淋巴管生成 

分 类 号：R734.2[医药卫生—肿瘤]