水稻功米3号高抗性淀粉性状的遗传分析  

Genetic Analysis of High Resistant Starch Characteristics for Rice Variety Gongmi 3(Oryza sativa ssp. indica)

作　　者：罗曦[1] 黄锦峰[1,2] 朱永生[1] 谢鸿光[1] 吴方喜[1] 张木清[2] 张建福[1] 谢华安[1] 

机构地区：[1]福建省农业科学院水稻研究所/农业部华南杂交水稻种质创新与分子育种重点实验室/福州国家水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/杂交水稻国家重点实验室华南基地/水稻国家工程实验室,福州350003 [2]福建农林大学,福州35000

出　　处：《农业生物技术学报》2014年第1期10-16,共7页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863)项目(No.2010AA101801;No.2010AA101804和No.2011AA10A101);国家水稻产业技术体系项目(No.CARS-01-11);福建省自然科学基金项目(No.2011J0901109);福建省财政专项-福建省农业科学院科技创新团队建设项目(No.CXTD-1-1301)

摘　　要：抗性淀粉具有降低糖尿病患者饭后血糖值、减少肠机能失调及结肠癌发病率、提供能量以及防止脂肪堆积等重要生理功能。为了了解水稻(Oryza sativa L.)中抗性淀粉含量这一性状的遗传特点,本研究以高抗淀粉水稻品种籼稻(indica)功米3号和粳稻(japonica)日本晴的正、反交组合F1及其自交后代F2、F2∶3群体为研究材料,分别测定各单株的抗性淀粉含量以分析其遗传特点。结果显示,正、反交组合的F1抗性淀粉含量及淀粉颗粒形态均表现出与相应母本相似的遗传现象,但是正、反交组合F2的抗性淀粉含量没有显著差异,而且F2∶3发生性状分离,说明这一性状受母性效应的影响。通过对功米3号/日本晴F2∶3群体的群分离体混合分析,在1号和6号染色体上分别筛选出3对和6对可能与抗性淀粉含量位点连锁的微卫星标记;利用QTL(quantitative trait locus)作图软件检测到3个抗性淀粉含量QTL(qRS6-1、sqRS6-2和qRS6-3),均分布在6号染色体上,其中qRS6-2和qRS6-3可解释表型变异分别为20.36%和40.13%,同时检测到1号和6号染色体之间存在着1对上位性QTLs。以上结果说明,抗性淀粉含量这一性状是由少数主效基因和多个微效基因以及非等位基因间互作所控制的数量性状(QTL)。以上结果为高抗性淀粉水稻育种的母本选择提供了理论依据,为精确定位抗性淀粉含量相关的主效基因提供了基础资料。Resistant starch has important physiological functions which reduce blood sugar levels of people with diabetes, reduce bowel dysfunction and colon cancer incidence of a disease, provide energy and prevent fat accumulation. In order to understand the genetic characteristics of resistant starch content in rice（Oryza sativa L.）, the content of resistant starch of reciprocal cross F~ and F2, F2：3 was analyzed derived from high resistant starch content variety Gongmi 3（indica） and low resistant starch content variety Nipponbare （japonica）. The results indicated that the content of resistant starch and the morphology of starch granule of reciprocal cross F~ were similar to their female parent, but their F2 had no significant difference in resistant starch content, the characteristics separation in F2： ~. So the content of resistant starch was significant by the influence of maternal effect. Three pairs of SSR markers from chromosome 1 and 6 pairs of SSR markers from chromosome 6 were screened possible linkage with resistant starch loci by bulked segregation analysis of F2：3 population of Gongmi3/Nipponbare. Three QTL loci（qRS6-1, sqRS6-2 and qRS6-3） were detected by QTL software. Among them, 20.3556% and 40.1349% phenotypic variation could be explained for the qRS6-2 and qRS6-3 of the total variation, respectively. Meanwhile, 1 epistasis effect was found between chromosome 1 and chromosome 6. The results demonstrated that resistant starch content was a quantitative trait controlled by a few main genes and several minor genes, the interactions of non-allelic genes： The above results provide theoretical basis for female parent choose of high resistant starch in rice breeding and make a solid foundation for fine mapping of main effect genes which associated with resistant starch content.

关 键 词：抗性淀粉含量 遗传分析 QTL定位 功米3号 水稻 

分 类 号：S512.1[农业科学—作物学]