白细胞介素-10基因修饰对大鼠肝细胞系BRL生长的影响  

Effect of Interleukin-10 Gene Modification on Rat Hepatocye Line BRL in vitro

作　　者：陈运新[1] 黄月红 张莉娟[1] 陈治新 王小众[1] 

机构地区：[1]福建医科大学附属协和医院消化内科,福州350001 [2]福建省消化研究所,福州350001

出　　处：《福建医科大学学报》2013年第5期271-273,共3页Journal of Fujian Medical University

基　　金：2012福建省临床医学重点专科基金;福建省自然基金(青年)(2010J05062;2010J01165);福建省卫生厅青年基金(2010-1-10)

摘　　要：目的观察白细胞介素-10(IL-10)基因修饰对大鼠肝细胞增殖、凋亡的影响,并筛选出表达可能受影响的细胞因子。方法体外培养大鼠肝细胞系BRL,分为对照组(C组),转染pcDNA3.0空质粒(P组),转染pcDNA3.0-rIL-10重组质粒(I组)。MTT法检测其增殖,流式细胞法检测其凋亡,抗体芯片法检测其细胞因子的表达。结果转染48h后,P组较C组增殖减少(P=0.000),凋亡增加(P=0.000);I组较P组增殖差别无统计学意义(P=0.279),但凋亡明显减少(P=0.000)。抗体芯片显示I组较P组除IL-10表达增加之外,血管表皮生长因子(VEGF)的表达下调。结论 IL-10基因治疗肝纤维化中,将肝细胞做为IL-10基因的表达细胞具有可行性。IL-10基因修饰后,肝细胞除了高表达IL-10之外,还通过下调VEGF的表达间接作用。bjective To observe the effect of interleukin-10 gene modification on the prolifera-tion ,apoptosis and cytokine expression of rat hepatocye in vitro . Method BRL cells ,a normal rat hepa-tocyte line cultured in vitro , were divided into three groups ,group Cwas control group ,group Pwere transfected with plasmid pcDNA 3 .0 and group I with plasmid pcDNA 3 .0-rIL-10 .MTT assay ,flow cyto-metric analysis and cytokine antibody arrays were used to detect on the proliferation ,apoptosis and cyto-kine differential expression of BRL cells . Results Transfected with plasmid pcDNA3 .0 ,proliferation of BRL cells was inhibited ,and apoptosis was increased . This increase of apoptosis was attenuated in BRL cells of group I . Compare with group P ,BRL cells of group I showed a lower expression of vascular en-dothelial growth factor ,in addition to higher expression of IL-10 . Conclusion Hepatocytes are possible to be the ideal target cells of IL-10 gene transfer in the IL-10 gene therapy of liver fibrosis . T ransfected with IL-10 gene ,hepatocytes played an inhibitory role on liver fibrogenesis not only by high IL-10 produc-tion ,but also by low regulation on expression of vascular endothelial growth factor probably .

关 键 词：白细胞介素10 肝细胞 基因 基因表达 基因疗法 细胞因子类 血管内皮生长因子类 

分 类 号：R575.2[医药卫生—消化系统]