ARHI基因mRNA和蛋白表达水平与胶质瘤恶性度相关性探讨  

The Relationship between Expression of ARHI mRNA and Protein and Malignant Grades of Glioma

作　　者：陈靖[1] 石松生[1] 张国良[1] 陈春美[1] 王春华[1] 

机构地区：[1]福建医科大学附属协和医院神经外科,福州350001

出　　处：《福建医科大学学报》2013年第5期279-283,共5页Journal of Fujian Medical University

基　　金：福建省自然科学基金青年人才项目(2013J05113)

摘　　要：目的探讨ARHI基因表达水平与人脑胶质瘤恶性度的相关性。方法采用RT-PCR和Western blot法检测10例正常脑组织、59例胶质瘤组织、4种胶质瘤细胞系(U251,SHG44,BT325,U87)中ARHI基因mRNA和蛋白的表达强度,结合临床病理资料统计分析。结果胶质瘤组织、细胞系中ARHI基因的表达水平显著低于正常脑组织,胶质瘤组织中ARHI的表达水平随肿瘤恶性程度增加而明显降低(P<0.01)。结论 ARHI基因mRNA和蛋白在胶质瘤中低表达,且表达水平与胶质瘤恶性程度负相关,提示ARHI基因低表达对胶质瘤的形成和恶性演变具有重要作用。Objective To investigate the relationship between ARHI expression and malignant grades of human brain glioma . Methods RT-PCR and Western blot methods were used to detect the ex-pression level of mRNA and protein of ARHI gene in 59 pairs of giloma samples ,10 normal brain tissues and 4 malignant glioma cell lines（U251 ,SHG44 ,BT325 and U87） . The implications between the expres-sion of ARHI and clinical-pathological factors were analyzed . Results It was found that the expression of ARHI in glioma tissues and glioma cell lines were significantly lower than that in normal brain tissues . The expressions of ARHI mRNA and protein decreased as the upgrading of glioma malignant grade fromⅠ to Ⅳ（P〈0 .01） . Conclusions The low expressions of ARHI mRNA and protein were founded in gli-oma and the expression levels were negatively correlated with the malignant grade of glioma . It was indi-cated that the low expression of ARHI might contribute to the occurrence and malignant transformation of glioma .

关 键 词：ρgtp结合蛋白质类 基因 神经胶质瘤 逆转录聚合酶链反应 蛋白质印迹法 RNA 信使 

分 类 号：R739.41[医药卫生—肿瘤]